2. Recombinant Proteins
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. CYP7A1 Protein, Human (His)

CYP7A1 Protein, Human (His)

Cat. No.: HY-P702798
COA Handling Instructions

CYP7A1 Protein, Human (His) is the recombinant human-derived CYP7A1, expressed by E. coli , with N-6*His labeled tag. The total length of CYP7A1 Protein, Human (His) is 504 a.a.,

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $155 In-stock
50 μg $300 In-stock
100 μg $480 In-stock
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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CYP7A1 Protein, Human (His) is the recombinant human-derived CYP7A1, expressed by E. coli , with N-6*His labeled tag. The total length of CYP7A1 Protein, Human (His) is 504 a.a.,
Species

Human
Source

E. coli
Tag

N-6*His
Accession

P22680 (M1-L504)
Gene ID

1581
Synonyms
Cytochrome P450 7A1; 24-hydroxycholesterol 7-alpha-hydroxylase; CYPVII; Cholesterol 7-alpha-hydroxylase
Molecular Weight

64 kDa
Purity

Greater than 90 % as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.
Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation

COA (219 KB)

Handling Instructions (2659 KB)

CYP7A1 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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