Cytosine deaminase/CodA Protein, E.coli (His-SUMO)

Cytosine deaminase (CodA) protein plays a key role in the pyrimidine salvage pathway by catalyzing the hydrolytic deamination of cytosine to uracil. This enzyme activity enables cells to efficiently utilize cytosine for pyrimidine nucleotide synthesis. Cytosine deaminase/CodA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Cytosine deaminase/CodA protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

Cytosine deaminase, encoded by the codA gene, is an enzyme that catalyzes the hydrolytic deamination of cytosine to uracil. This enzymatic activity is a key step in the pyrimidine salvage pathway, enabling the cell to utilize cytosine for the synthesis of pyrimidine nucleotides. In addition to its primary function, Cytosine deaminase also exhibits deaminase activity towards isoguanine, an oxidation product of adenine in DNA, and isocytosine. Furthermore, the enzyme displays a secondary activity, converting 5-fluorocytosine (5FC) to 5-fluorouracil (5FU). This ability to transform a non-cytotoxic precursor, 5FC, into a cytotoxic chemotherapeutic agent, 5FU, highlights the potential therapeutic applications of Cytosine deaminase in cancer treatment.

E.coli

E. coli

N-His;N-SUMO

P25524 (S2-R427)

944966  [NCBI]

SNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR

Approximately 63.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.