DCK/Deoxycytidine kinase Protein, Human (His-T7)

DCK/deoxycytidine kinase protein can effectively phosphorylate deoxyribonucleosides, including deoxycytidine, deoxyguanosine and deoxyadenosine, showing broad substrate specificity and no chiral selectivity. As an indispensable enzyme, DCK plays a crucial role in phosphorylating nucleoside analogues, which is critical in antiviral and chemotherapeutic treatments. DCK/Deoxycytidine kinase Protein, Human (His-T7) is the recombinant human-derived DCK/Deoxycytidine kinase protein, expressed by E. coli , with N-6*His, N-T7 labeled tag. The total length of DCK/Deoxycytidine kinase Protein, Human (His-T7) is 260 a.a., with molecular weight of ~32.0 kDa.

DCK, known as deoxycytidine kinase, demonstrates its enzymatic prowess by effectively phosphorylating deoxyribonucleosides, including deoxycytidine, deoxyguanosine, and deoxyadenosine. With broad substrate specificity, this kinase does not exhibit selectivity based on the chirality of the substrate. Its significance extends to being an indispensable enzyme for the phosphorylation of various nucleoside analogs commonly utilized as antiviral and chemotherapeutic agents. The ability of DCK to catalyze the phosphorylation of nucleosides plays a crucial role in cellular processes and contributes to the therapeutic efficacy of nucleoside analog-based treatments.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-T7

P27707 (M1-L260)

1633  [NCBI]

MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL

Approximately 32.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of PBS, 50% Glycerol, 1 mM TCEP, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.