Delta-like protein 4/DLL4 Protein, Human (Biotinylated, HEK293, His)

Delta-like protein 4 (DLL4) functions as a Notch ligand and activates NOTCH1 and NOTCH4 in the Notch signaling pathway. It regulates angiogenesis by inhibiting endothelial cell proliferation, migration, and angiogenesis. Delta-like protein 4/DLL4 Protein, Human (Biotinylated, HEK293, His) is the recombinant human-derived Delta-like protein 4/DLL4 protein, expressed by HEK293 , with C-His labeled tag. The total length of Delta-like protein 4/DLL4 Protein, Human (Biotinylated, HEK293, His) is 498 a.a., with molecular weight of 60-70 kDa.

Delta-like protein 4 (DLL4) is a key participant in the Notch signaling pathway, acting as a Notch ligand with functional implications. It specifically activates NOTCH1 and NOTCH4, contributing to the intricate cellular communication within this signaling cascade. Beyond its role in Notch signaling, DLL4 emerges as a crucial regulator of angiogenesis, exerting a negative influence on endothelial cell proliferation, migration, and angiogenic sprouting. The protein's indispensability extends to retinal progenitor proliferation, where it plays a vital role in suppressing rod fates in late retinal progenitors and ensuring the proper generation of diverse retinal cell types. Additionally, during spinal cord neurogenesis, DLL4 inhibits V2a interneuron fate, adding another layer to its multifaceted functions. The molecular interactions involving DLL4 include its engagement with NOTCH4 and NOTCH1, facilitated by specific domains in DLL4 and NOTCH1, respectively.

Immobilized Anti-DLL4 Antibody, hFc Tag at 1 μg/mL (100 μl/Well) on the plate. Dose response curve for Biotinylated Human DLL4, His Tag with the EC₅₀ of 0.3-0.49 μg/mL determined by ELISA.

Human

HEK293

C-His

Q9NR61 (S27-P524)

54567  [NCBI]

SGVFQLQLQEFINERGVLASGRPCEPGCRTFFRVCLKHFQAVVSPGPCTFGTVSTPVLGTNSFAVRDDSSGGGRNPLQLPFNFTWPGTFSLIIEAWHAPGDDLRPEALPPDALISKIAIQGSLAVGQNWLLDEQTSTLTRLRYSYRVICSDNYYGDNCSRLCKKRNDHFGHYVCQPDGNLSCLPGWTGEYCQQPICLSGCHEQNGYCSKPAECLCRPGWQGRLCNECIPHNGCRHGTCSTPWQCTCDEGWGGLFCDQDLNYCTHHSPCKNGATCSNSGQRSYTCTCRPGYTGVDCELELSECDSNPCRNGGSCKDQEDGYHCLCPPGYYGLHCEHSTLSCADSPCFNGGSCRERNQGANYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLNRGPSRMCRCRPGFTGTYCELHVSDCARNPCAHGGTCHDLENGLMCTCPAGFSGRRCEVRTSIDACASSPCFNRATCYTDLSTDTFVCNCPYGFVGSRCEFPVGLP

60-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB,500 mM NaCl, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.