ECSIT Protein, Human (His)

ECSIT protein is an adapter protein in Toll-like receptors, IL-1, and antiviral signaling that crucially activates NF-kappa-B by complexing with TRAF6 and TAK1/MAP3K7, leading to TAK1/MAP3K7 activation and subsequent IKK activation. Ubiquitinated ECSIT interacts with RELA and NFKB1 and translocates to induce NF-kappa-B-dependent gene expression. ECSIT Protein, Human (His) is the recombinant human-derived ECSIT protein, expressed by E. coli , with N-His labeled tag.

ECSIT protein functions as an adapter protein in various signaling pathways, including Toll-like receptors (TLRs), interleukin-1 (IL-1) pathways, and innate antiviral induction signaling. It plays a pivotal role in activating NF-kappa-B by forming a signal complex with TRAF6 and TAK1/MAP3K7, ultimately leading to the activation of TAK1/MAP3K7 and subsequent IKK activation. Upon ubiquitination, ECSIT interacts with dissociated RELA and NFKB1 proteins, translocating to the nucleus and inducing NF-kappa-B-dependent gene expression. In the context of the innate antiviral immune response, ECSIT bridges pattern recognition receptors RIGI and MDA5/IFIT1 to the MAVS complex at the mitochondrion. Additionally, ECSIT promotes the proteolytic activation of MAP3K1, participates in the BMP signaling pathway, and is essential for normal embryonic development. Furthermore, as part of the MCIA complex, ECSIT contributes to the assembly of mitochondrial complex I.

Human

E. coli

N-His

Q9BQ95-1 (E246-S431)

51295  [NCBI]

QVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINEVEEGPVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEEDDNLQRQQQGQS

Approximately 25 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.