ENTPD5 Protein, Human (sf9, His)

The ENTPD5 protein is located in the lumen of the endoplasmic reticulum and plays a crucial role in glycoprotein processing by preferentially hydrolyzing nucleoside diphosphates, including GDP, IDP, and UDP. Its enzymatic activity ensures the clearance of UDP, an end-product feedback inhibitor of UDP-Glc:glycoprotein glucosyltransferase. ENTPD5 Protein, Human (sf9, His) is the recombinant human-derived ENTPD5 protein, expressed by Sf9 insect cells , with C-His labeled tag.

ENTPD5 protein exhibits its enzymatic activity by preferentially hydrolyzing nucleoside diphosphates, showing a distinct preference for GDP, IDP, and UDP over ADP and CDP. Operating within the endoplasmic reticulum lumen, ENTPD5 plays a crucial role in the clearance of UDP, which functions as an end-product feedback inhibitor for UDP-Glc:glycoprotein glucosyltransferases. The hydrolyzed UMP is transported back to the cytosol through an UDP-sugar antiporter, where it is consumed to regenerate UDP-glucose. This mechanism positively regulates protein reglucosylation by ensuring the removal of UDP from the ER lumen and facilitating the regeneration of UDP-glucose. The process of protein reglucosylation, governed by ENTPD5, is integral to maintaining proper glycoprotein folding and upholding quality control within the endoplasmic reticulum.

Human

Sf9 insect cells

C-His

O75356 (V21-H428)

957  [NCBI]

Approximately 45 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.