EphA1 Protein, Mouse (HEK293, His)

The EphA1 protein is a receptor tyrosine kinase that promiscuously binds to ephrin-A ligands on neighboring cells, initiating contact-dependent bidirectional signaling. The forward signal originates from the receptor and the reverse signal originates from the ephrin ligand. EphA1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphA1 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphA1 Protein, Mouse (HEK293, His) is 522 a.a., with molecular weight of ~68 kDa.

The EphA1 protein, a receptor tyrosine kinase, engages in promiscuous binding to membrane-bound ephrin-A family ligands on adjacent cells, initiating contact-dependent bidirectional signaling. The downstream pathway originating from the receptor is known as forward signaling, while the pathway downstream of the ephrin ligand is termed reverse signaling. EphA1 exhibits low-affinity binding to EFNA3 and EFNA4 and high-affinity binding to EFNA1, which likely constitutes its cognate/functional ligand. Upon activation by EFNA1, EphA1 induces cell attachment to the extracellular matrix, inhibiting cell spreading and motility through the regulation of ILK and downstream RHOA and RAC. Additionally, EphA1 plays a role in angiogenesis and regulates cell proliferation. It may also be involved in apoptosis processes, contributing to its diverse cellular functions.

Immobilized rmEphA1 at 2 µg/mL (100 µL/well) can bind Biotinylated rmEphrin-A1. The ED₅₀ for this effect is 28.67 ng/mL.

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  Immobilized rmEphA1 at 2 µg/mL (100 µL/well) can bind Biotinylated rmEphrin-A1. The ED₅₀ for this effect is 28.67 ng/mL.

Mouse

HEK293

C-His

Q60750 (E27-E548)

13835  [NCBI]

EEVTLMDTSTAQGELGWLLDPPETGWSEVQQMLNGTPLYMYQDCPIQEGGDTDHWLRSNWIYRGEEASRIYVELQFTVRDCKSFPGGAGPLGCKETFNLFYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLASGSVKLNVERCSLGHLTRRGLYLAFHNPGSCVALVSVRVFYQRCAETVHGLAHFPDTLPGPGGLVEVAGTCLSHAQISLGSSGTPRMHCSPDGEWLVPVGQCQCEPGYEESSGNVGCTACPTGFYRVDMNTLRCLKCPQHSIAESEGSTICTCENGHYRAPGEGPQVACTRPPSAPQNLSFSTSGTQLSLRWEPPRDTGGRHDIRYSVECLQCRGIAQDGGPCQPCGKGVHFSPAASGLTTSTVQVQGLEPYANYTFTVKSQNRVSGLDSSSPSSASLSINMGHAESLSGLSLKLVKKEPRQLELTWAGSRPRNPGGNLSYELHVLNQDEEWHQMVLEPRVLLTKLQPDTTYIVRVRTLTPLGPGPFSPDHEFRTSPPVSRSLTGGE

Approximately 68 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.