EphA2 Protein, Human (sf9, His-GST)

EphA2 protein is a receptor tyrosine kinase that participates in bidirectional signaling with various ephrin A ligands, affecting cell migration, adhesion, proliferation and differentiation. It regulates cell adhesion and differentiation by interacting with DSG1 and inhibiting the ERK1/ERK2 signaling pathway. EphA2 Protein, Human (sf9, His-GST) is the recombinant human-derived EphA2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of EphA2 Protein, Human (sf9, His-GST) is 392 a.a., with molecular weight of ~62 kDa.

EphA2 protein is a receptor tyrosine kinase that interacts with various membrane-bound ephrin-A family ligands on neighboring cells, leading to bidirectional signaling. This receptor is involved in regulating cell migration, adhesion, proliferation, and differentiation through forward and reverse signaling pathways. It plays a role in cell adhesion and differentiation by interacting with DSG1/desmoglein-1 and inhibiting the ERK1/ERK2 signaling pathway. Additionally, EphA2 protein may be involved in UV radiation-induced apoptosis and stimulate chemotactic cell migration independently of ligand binding. During development, it contributes to pattern formation and the development of fetal tissues, including angiogenesis, hindbrain development, and mammary gland morphogenesis. EphA2 protein also interacts with Ephrin-A5/EFNA5 to regulate lens fiber cells' shape and interactions, crucial for maintaining lens transparency. Moreover, it plays a role in bone remodeling by regulating osteoclastogenesis and osteoblastogenesis through its interaction with Ephrin-A2/EFNA2. Notably, EphA2 protein acts as a receptor for hepatitis C virus (HCV) in hepatocytes, facilitating viral cell entry by promoting the formation of CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins.

The specific activity was determined to be 50 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Human

Sf9 insect cells

N-His;N-GST

P29317 (L585-I976)

1969  [NCBI]

LKTYVDPHTYEDPNQAVLKFTTEIHPSCVTRQKVIGAGEFGEVYKGMLKTSSGKKEVPVAIKTLKAGYTEKQRVDFLGEAGIMGQFSHHNIIRLEGVISKYKPMMIITEYMENGALDKFLREKDGEFSVLQLVGMLRGIAAGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSFGIVMWEVMTYGERPYWELSNHEVMKAINDGFRLPTPMDCPSAIYQLMMQCWQQERARRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPSTSGSEGVPFRTVSEWLESIKMQQYTEHFMAAGYTAIEKVVQMTNDDIKRIGVRLPGHQKRIAYSLLGLKDQVNTVGIPI

Approximately 62 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 3 mM DTT, pH 8.5, 10% gly

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.