ERG11 Protein, Saccharomyces cerevisiae (GST)

ERG11 Proteinas is a sterol 14α-demethylase, a key enzyme in the ergosterol biosynthetic pathway, particularly in the late pathway that occurs in the endoplasmic reticulum membrane. ERG11 catalyzes the three-step removal of the 14α-methyl group from lanosterol, releasing it as a formate, resulting in the conversion of the sterol into a key intermediate in ergosterol biosynthesis. ERG11 Protein, Saccharomyces cerevisiae (GST) is the recombinant ERG11 protein, expressed by E. coli , with N-GST labeled tag. The total length of ERG11 Protein, Saccharomyces cerevisiae (GST) is 20 a.a., with molecular weight of ~29.1 kDa.

ERG11 protein, a sterol 14alpha-demethylase, is a crucial enzyme in the third module of the ergosterol biosynthesis pathway, playing a pivotal role in fungal membranes where ergosterol serves as the major sterol component with diverse functions. The third module, known as the late pathway, involves consecutive reactions primarily occurring in the endoplasmic reticulum (ER) membrane, ultimately leading to ergosterol synthesis. Initiating from lanosterol, ERG11 catalyzes a three-step oxidative removal of the 14alpha-methyl group (C-32) of the sterol, releasing it in the form of formate. This process results in the conversion of the sterol to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol, a critical intermediate in ergosterol biosynthesis. Interestingly, ERG11 exhibits the ability to demethylate substrates not intrinsic to yeast, including eburicol and 24,25-dihydrolanosterol, highlighting its versatility in sterol modification.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Others

E. coli

N-GST

P10614 (M1-S20)

856398  [NCBI]

MSATKSIVGEALEYVNIGLS

Approximately 29.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.