Erythropoietin receptor/EpoR Protein, Human (HEK293 , Fc)

The EPOR Protein functions as a receptor for erythropoietin, mediating erythroblast proliferation and differentiation upon EPO stimulation. The heterodimer triggers the JAK2/STAT5 signaling cascade and, in certain cell types, activates STAT1, STAT3, and the LYN tyrosine kinase. Additionally, isoform EPOR-T acts as a dominant-negative receptor, modulating EPOR-mediated signaling pathways. Erythropoietin receptor/EpoR Protein, Human (HEK293, Fc) is the recombinant human-derived Erythropoietin receptor/EpoR protein, expressed by HEK293 , with C-hFc labeled tag.

The EPOR serves as the receptor for erythropoietin (EPO), playing a crucial role in mediating EPO-induced erythroblast proliferation and differentiation. Upon stimulation by EPO, the EPOR component of the heterodimer undergoes dimerization, initiating the JAK2/STAT5 signaling cascade. In certain cell types, this heterodimeric receptor complex can additionally activate STAT1 and STAT3, and may participate in the activation of the LYN tyrosine kinase. Notably, the isoform EPOR-T acts as a dominant-negative receptor, modulating and attenuating EPOR-mediated signaling pathways. The intricate interplay within the EPORic complex underscores its significance in regulating cellular responses to EPO stimulation.

1. Measured by its binding ability in a functional ELISA. Immobilized EPOR (hFc Tag) at 2 μg/mL (100 μL/well) can bind Cynomolgus Erythropoietin-His, the EC₅₀ of Cynomolgus Erythropoietin-His is 4-20 ng/mL.
2. Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 1-4 ng/mL in the presence of 0.1 U/mL Recombinant Human EPO.

Human

HEK293

C-hFc

P19235-1/NP_000112.1 (A25-P250)

2057  [NCBI]

55-60 kDa

Greater than 90% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of PBS, 8% sucrose, 0.5% Tween-20, pH 7.4

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.