Ezrin/EZR Protein, Human

Ezrin (EZR) plays a crucial role in connecting major cytoskeletal structures to the plasma membrane and is particularly critical for the development of microvilli and membrane ruffles in epithelial cells. Ezrin cooperates with PLEKHG6 and is essential for normal macropinocytosis. Ezrin/EZR Protein, Human is the recombinant human-derived Ezrin/EZR protein, expressed by E. coli , with tag free. The total length of Ezrin/EZR Protein, Human is 586 a.a., with molecular weight of ~82.0 kDa.

Ezrin (EZR) likely plays a crucial role in establishing connections between major cytoskeletal structures and the plasma membrane. Particularly in epithelial cells, it is essential for the development of microvilli and membrane ruffles on the apical pole. Additionally, in collaboration with PLEKHG6, Ezrin is necessary for the normal process of macropinocytosis. The protein exhibits a head-to-tail association, bringing together its N-terminal and C-terminal halves, resulting in a closed conformation representing the inactive form. In this state, Ezrin is unable to bind to actin or the membrane, suggesting a regulatory mechanism for its functional activation.

Human

E. coli

Tag Free

P15311 (M1-L586)

7430  [NCBI]

MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL

Approximately 82.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Suppliedas a 0.2 μm filtered solution of 10 mM Hepes, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.