FGFR-1 beta Protein, Human (HEK293, hFc)

FGFR-1 beta is a member of the FGFR family that interacts with fibroblast growth factors and regulates mitosis and differentiation. It specifically binds acidic and basic fibroblast growth factors and is involved in limb induction. FGFR-1 beta Protein, Human (HEK293, hFc) is the recombinant human-derived FGFR-1 beta protein, expressed by HEK293 , with C-hFc labeled tag. The total length of FGFR-1 beta Protein, Human (HEK293, hFc) is 264 a.a., with molecular weight of 97.5-110 kDa.

The FGFR-1 beta protein is a highly conserved member of the fibroblast growth factor receptor (FGFR) family, sharing a similar amino acid sequence with other members. FGFR family members exhibit different affinities for ligands and are distributed across various tissues. The full-length protein consists of an extracellular region with three immunoglobulin-like domains, a hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion interacts with fibroblast growth factors, initiating downstream signals that regulate mitogenesis and differentiation. FGFR-1 beta specifically binds to both acidic and basic fibroblast growth factors and plays a role in limb induction. Mutations in this gene have been associated with several syndromes and disorders, including Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are linked to stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Various alternatively spliced variants encoding different protein isoforms have been identified, although not all variants have been fully characterized. The FGFR-1 beta protein exhibits ubiquitous expression in multiple tissues, including the ovary, fat, and 25 other tissues.

Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 1.096 ng/mL in the presence of 1ng/mL human FGF-1, corresponding to a specific activity is 9.12×10^5 units/mg.

Human

HEK293

C-hFc

NP_075594.1 (R22-E285)

2260  [NCBI]

RPSPTLPEQDALPSSEDDDDDDDSSSEEKETDNTKPNPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLE

97.5-110 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.