FGFR-4 Protein, Human (HEK293, Fc)

The leptin R/LEPR protein is a receptor protein that binds to the hormone leptin. It plays a vital role in regulating energy balance and body weight. FGFR-4 Protein, Human (HEK293, Fc) is the recombinant human-derived FGFR-4 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of FGFR-4 Protein, Human (HEK293, Fc) is 348 a.a., with molecular weight of 95-112 kDa.

The FGFR-4 Protein serves as a tyrosine kinase and cell surface receptor for fibroblast growth factors, playing a pivotal role in regulating multiple pathways, including cell proliferation, differentiation, migration, lipid metabolism, bile acid biosynthesis, vitamin D metabolism, glucose uptake, and phosphate homeostasis. Structurally, the protein comprises an extracellular region with three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. Through its extracellular portion, which interacts with fibroblast growth factors, FGFR-4 initiates downstream signaling cascades that ultimately influence mitogenesis and differentiation. The gene demonstrates broad expression, with elevated levels observed in the lung (RPKM 16.7), kidney (RPKM 12.5), and 15 other tissues, underscoring its potential significance in diverse physiological contexts across various organs.

1. Measured by its ability to inhibit FGF acidic (aFGF / FGF1) dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts and the ED₅₀ is typically 4-25 ng/mL.
2. Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ of this effect is 2.694 ng/mL in the presence of 1ng/mL FGF-1, corresponding to a specific activity is 3.71×10⁵ units/mg.

• 
  Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ of this effect is 2.694 ng/ml in the presence of 1ng/mL FGF-1, corresponding to a specific activity is 3.71×10⁵ units/mg.

Human

HEK293

C-hFc

NP_002002.3 (L22-D369)

2264  [NCBI]

LEASEEVELEPCLAPSLEQQEQELTVALGQPVRLCCGRAERGGHWYKEGSRLAPAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMIVLQNLTLITGDSLTSSNDDEDPKSHRDPSNRHSYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPTPTIRWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENAVGSIRYNYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTADINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDPTWTAAAPEARYTD

95-112 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.