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  3. FIP1L1 Protein, Human (Sf9, His, GST)

FIP1L1 Protein, Human (Sf9, His, GST)

Cat. No.: HY-P701686
Handling Instructions

The FIP1L1 protein is an important component of the CPSF complex and is responsible for the formation of the 3' end of pre-mRNA. FIP1L1 Protein, Human (Sf9, His, GST) is the recombinant human-derived FIP1L1 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of FIP1L1 Protein, Human (Sf9, His, GST) is 593 a.a., .

For research use only. We do not sell to patients.

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Description

The FIP1L1 protein is an important component of the CPSF complex and is responsible for the formation of the 3' end of pre-mRNA. FIP1L1 Protein, Human (Sf9, His, GST) is the recombinant human-derived FIP1L1 protein, expressed by Sf9 insect cells , with N-8*His, N-GST labeled tag. The total length of FIP1L1 Protein, Human (Sf9, His, GST) is 593 a.a., .

Background

FIP1L1, a vital component of the cleavage and polyadenylation specificity factor (CPSF) complex, plays a crucial role in pre-mRNA 3'-end formation by recognizing the AAUAAA signal sequence and facilitating interactions with poly(A) polymerase and other factors, leading to cleavage and poly(A) addition. Notably, FIP1L1 contributes to poly(A) site recognition, stimulates poly(A) addition, and binds to U-rich RNA sequence elements surrounding the poly(A) site. Its presence in the CPSF complex, alongside CPSF1, CPSF2, CPSF3, and CPSF4, highlights its integral role in the intricate process of pre-mRNA processing. FIP1L1 forms specific interactions with various partners, including CPSF1, CPSF4, CSTF2, and CSTF3, influencing the overall functionality of the CPSF complex. Moreover, FIP1L1 engages in direct interactions with AHCYL1 when phosphorylated, linking AHCYL1 to the CPSF complex and RNA. Additionally, it interacts with NUDT21/CPSF5 in a sequence-specific manner. Furthermore, FIP1L1 engages in interactions with CPSF6 and CPSF7, mediated by specific domains, contributing to the coordination between the CFIm and CPSF complexes. The interaction with PAPOLA is also noted, potentially modulated by its association with AHCYL1 (By similarity).

Species

Human

Source

Sf9 insect cells

Tag

N-8*His;N-GST

Accession

Q6UN15 (S2-E594)

Gene ID

81608

Molecular Construction
N-term
8*His-GST
FIP1L1 (S2-E594)
Accession # Q6UN15
C-term
Synonyms
FIP1L1; Pre-mRNA 3'-end-processing factor FIP1; hFip1; FIP1-like 1 protein; Factor interacting with PAP; Rearranged in hypereosinophilia
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

FIP1L1 Protein, Human (Sf9, His, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FIP1L1 Protein, Human (Sf9, His, GST)
Cat. No.:
HY-P701686
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