GAD2/GAD65 Protein, Mouse (sf9)

Cat. No.: HY-P75178: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Protein solubilization and storage

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GAD2/GAD65 Protein is a key enzyme in neurotransmitter metabolism, specifically catalyzing the production of gamma-aminobutyric acid (GABA). Meanwhile, GAD2 also has glutamate binding activity, glutamate decarboxylase activity, and pyridoxal phosphate binding activity. GAD2 is expressed in several structures, including the genitourinary system, the gastrointestinal nervous system, and sensory organs. It can be used to study epilepsy and type 1 diabetes. GAD2/GAD65 Protein, Mouse (sf9) is the recombinant mouse-derived GAD2/GAD65 protein, expressed by Sf9 insect cells , with tag free. The total length of GAD2/GAD65 Protein, Mouse (sf9) is 585 a.a., with molecular weight of ~58 kDa.

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Size	Price	Stock	Quantity
5 μg	USD 65	In-stock	Estimated Time of Arrival: April 8
10 μg	USD 105	In-stock	Estimated Time of Arrival: April 8
50 μg	USD 300	In-stock	Estimated Time of Arrival: April 8
100 μg	USD 480	In-stock	Estimated Time of Arrival: April 8
> 100 μg		Get quote

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Description	GAD2/GAD65 Protein is a key enzyme in neurotransmitter metabolism, specifically catalyzing the production of gamma-aminobutyric acid (GABA). Meanwhile, GAD2 also has glutamate binding activity, glutamate decarboxylase activity, and pyridoxal phosphate binding activity. GAD2 is expressed in several structures, including the genitourinary system, the gastrointestinal nervous system, and sensory organs. It can be used to study epilepsy and type 1 diabetes. GAD2/GAD65 Protein, Mouse (sf9) is the recombinant mouse-derived GAD2/GAD65 protein, expressed by Sf9 insect cells , with tag free. The total length of GAD2/GAD65 Protein, Mouse (sf9) is 585 a.a., with molecular weight of ~58 kDa.
Background	Glutamate decarboxylase 2 (GAD2) is a key enzyme in neurotransmitter metabolism, specifically catalyzing the production of gamma-aminobutyric acid (GABA). GABA is a crucial inhibitory neurotransmitter in the central nervous system, playing a pivotal role in regulating neuronal excitability and maintaining the balance between excitation and inhibition in the brain. As the catalyst for GABA synthesis, GAD2 plays a fundamental role in modulating neurotransmission and influencing various physiological processes, highlighting its importance in the intricate network of neural signaling within the nervous system. Meanwhile, GAD2 also has glutamate binding activity, glutamate decarboxylase activity, and pyridoxal phosphate binding activity. GAD2 is expressed in several structures, including the genitourinary system, the gastrointestinal nervous system, and sensory organs. In addition, GAD2 can be used to study epilepsy and type 1 diabetes^[1].
Biological Activity	The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Species	Mouse
Source	Sf9 insect cells
Tag	Tag Free
Accession	Q548L4 (N-G&P, M1-L585)
Gene ID	14417 [NCBI]
Molecular Construction	N-term Gly-Pro GAD2 (M1-L585) Accession # Q548L4 C-term
Synonyms	Glutamate decarboxylase 2; GAD-65; GAD2
Molecular Weight	Approximately 58 kDa
Purity	Greater than 90% as determined by reducing SDS-PAGE
Appearance	Solution
Formulation	Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Glycerol, 3 mM DTT, pH 8.0.
Endotoxin Level	<1 EU/μg, determined by LAL method.
Reconstitution	N/A.
Storage & Stability	Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping	Shipping with dry ice.
Documentation	Select Batch: Data Sheet (232 KB) SDS (251 KB) COA (206 KB) Handling Instructions (2659 KB)

GAD2/GAD65 Protein, Mouse (sf9) Related Classifications

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?
1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.
How should solution-form recombinant proteins be stored?
1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that
Why is it necessary to add carrier proteins?
Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.
Carrier protein types and options?
In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

Reconstitution Calculator
Dilution Calculator
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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial)	=	Mass (in vial)	÷	Desired Reconstitution Concentration
	=		÷

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 10⁶ ÷ Biological Activity (ED₅₀)

Specific Activity (Unit/mg)	=	10⁶	÷	Biological Activity (ED₅₀)
Unit/mg	=	10⁶	÷	ng/mL

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GAD2/GAD65 Protein, Mouse (sf9)

GAD2/GAD65 Protein, Mouse (sf9) Featured Recommendations:

Biological Activity

Technical Parameters

Properties

Documentation

GAD2/GAD65 Protein, Mouse (sf9) Related Classifications

Reconstitution Calculator

Dilution Calculator

Specific Activity Calculator

The reconstitution calculator equation

The dilution calculator equation

The specific activity calculator equation

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