Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His)

Gamma-hemolysin component C (HLgC) acts as a toxin, forming cell membrane pores with hemolytic and leukotoxic activities. The toxicity of HlgC requires sequential binding and cooperative association with S- and F-class components to form heterooligomeric complexes. Gamma-hemolysin component C Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived Gamma-hemolysin component C protein, expressed by P. pastoris , with N-His labeled tag.

Gamma-hemolysin component C (HlgC) is a toxin known for its mechanism of action involving the formation of pores in the cell membrane, resulting in both hemolytic and leucotoxic activities. The toxicity of HlgC necessitates a sequential binding and synergistic association of a class S and a class F component, giving rise to the formation of heterooligomeric complexes. In particular, HlgC, belonging to the class S, associates with HlgB, a class F component, forming a CB toxin. This cooperative interaction underscores the intricate molecular processes employed by HlgC to exert its toxic effects on target cells.

Staphylococcus aureus

P. pastoris

N-His

Q7A3S2 (A30-N315)

/

ANDTEDIGKGSDIEIIKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKKTNHVKAMRWPFQYNIGLKTNDKYVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSFNYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFATESGQKSAFDSDLFVGYKPHSKDPRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSSDTSEFEITYGRNMDVTHAIKRSTHYGNSYLDGHRVHNAFVNRNYTVKYEVNWKTHEIKVKGQN

Approximately 34.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.