1. Recombinant Proteins
  2. Viral Proteins
  3. glycoprotein E/gE Protein, HHV-2 (Cell-Free, His)

glycoprotein E/gE Protein, HHV-2 (Cell-Free, His)

Cat. No.: HY-P702293
Handling Instructions

Glycoprotein E/gE proteins bind to gI and promote viral spread in epithelial cells by sorting viral particles to cell junctions. It is able to spread to neighboring cells through receptor interactions. glycoprotein E/gE Protein, HHV-2 (Cell-Free, His) is the recombinant Virus-derived glycoprotein E/gE protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of glycoprotein E/gE Protein, HHV-2 (Cell-Free, His) is 525 a.a., with molecular weight of 60.1 kDa.

For research use only. We do not sell to patients.

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Description

Glycoprotein E/gE proteins bind to gI and promote viral spread in epithelial cells by sorting viral particles to cell junctions. It is able to spread to neighboring cells through receptor interactions. glycoprotein E/gE Protein, HHV-2 (Cell-Free, His) is the recombinant Virus-derived glycoprotein E/gE protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of glycoprotein E/gE Protein, HHV-2 (Cell-Free, His) is 525 a.a., with molecular weight of 60.1 kDa.

Background

The glycoprotein E/gE protein, forming a heterodimer with gI, plays a crucial role in the cell-to-cell spread of the virus in epithelial cells by facilitating the sorting of nascent virions to cell junctions. Once positioned at these junctions, virus particles can rapidly disseminate to neighboring cells through interactions with cellular receptors that accumulate in these regions. Notably, gE/gI is implicated in basolateral spread in polarized cells. In neuronal cells, this heterodimer is essential for the anterograde spread of the infection throughout the host nervous system, working in tandem with US9 to sort and transport viral structural components toward axon tips. Beyond its role in virus propagation, gE/gI serves as a receptor for the Fc part of host IgG. Under acidic pH conditions, dissociation of gE/gI from IgG occurs, suggesting involvement in anti-HSV antibodies bipolar bridging. This process may lead to intracellular endocytosis and degradation, potentially interfering with host IgG-mediated immune responses. The diverse functions of the glycoprotein E/gE protein underscore its pivotal role in the complex life cycle and immune evasion strategies of the herpes simplex virus.

Species

Virus

Source

E. coli Cell-free

Tag

N-10*His

Accession

P89475 (A21-W545)

Gene ID

/

Molecular Construction
N-term
10*His
HHV-2 gE (A21-W545)
Accession # P89475
C-term
Synonyms
Envelope glycoprotein E; gE-2
AA Sequence

AAPRTSWKRVTSGEDVVLLPAPAERTRAHKLLWAAEPLDACGPLRPSWVALWPPRRVLETVVDAACMRAPEPLAIAYSPPFPAGDEGLYSELAWRDRVAVVNESLVIYGALETDSGLYTLSVVGLSDEARQVASVVLVVEPAPVPTPTPDDYDEEDDAGVTNARRSAFPPQPPPRRPPVAPPTHPRVIPEVSHVRGVTVHMETLEAILFAPGETFGTNVSIHAIAHDDGPYAMDVVWMRFDVPSSCADMRIYEACLYHPQLPECLSPADAPCAVSSWAYRLAVRSYAGCSRTTPPPRCFAEARMEPVPGLAWLASTVNLEFQHASPQHAGLYLCVVYVDDHIHAWGHMTISTAAQYRNAVVEQHLPQRQPEPVEPTRPHVRAPHPAPSARGPLRLGAVLGAALLLAALGLSAWACMTCWRRRSWRAVKSRASATGPTYIRVADSELYADWSSDSEGERDGSLWQDPPERPDSPSTNGSGFEILSPTAPSVYPHSEGRKSRRPLTTFGSGSPGRRHSQASYPSVLW

Molecular Weight

60.1 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

glycoprotein E/gE Protein, HHV-2 (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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glycoprotein E/gE Protein, HHV-2 (Cell-Free, His)
Cat. No.:
HY-P702293
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