1. Recombinant Proteins
  2. Receptor Proteins
  3. G-Protein-Coupled Receptors (GPCRs)
  4. GRM5 Protein, Human (His)

The GRM5 protein is a G protein-coupled glutamate receptor that undergoes ligand-induced conformational changes and activates signaling cascades through G proteins, affecting downstream effectors. This cascade includes the phosphatidylinositol-calcium second messenger system and induces calcium-activated chloride currents. GRM5 Protein, Human (His) is the recombinant human-derived GRM5 protein, expressed by Sf9 insect cells , with C-10*His labeled tag. ,

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Description

The GRM5 protein is a G protein-coupled glutamate receptor that undergoes ligand-induced conformational changes and activates signaling cascades through G proteins, affecting downstream effectors. This cascade includes the phosphatidylinositol-calcium second messenger system and induces calcium-activated chloride currents. GRM5 Protein, Human (His) is the recombinant human-derived GRM5 protein, expressed by Sf9 insect cells , with C-10*His labeled tag. ,

Background

The GRM5 Protein functions as a G-protein coupled receptor for glutamate, undergoing a conformational change upon ligand binding that initiates signaling through guanine nucleotide-binding proteins (G proteins), ultimately modulating downstream effectors. This signaling cascade activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Integral to the regulation of synaptic plasticity and modulation of neural network activity, GRM5 plays a crucial role in shaping the dynamic responses of the central nervous system. The interaction of the PPXXF motif with HOMER1, HOMER2, and HOMER3 highlights its involvement in intracellular signaling events. Additionally, GRM5 interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3, TAMALIN, and NCDN, with isoform 2 specifically interacting with NECAB2. Further interaction occurs with CAMK2A, emphasizing the intricate network through which GRM5 contributes to the regulation of neural function.

Species

Human

Source

Sf9 insect cells

Tag

C-10*His

Accession

P41594 (P569-K678, E579A, N667Y, I669A, G675M)-T4L-P41594 (K679-A836, T742A, S753A)

Gene ID

2915

Synonyms
GRM5; Metabotropic glutamate receptor 5; mGluR5
Molecular Weight

49.2 KDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

GRM5 Protein, Human (His) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
GRM5 Protein, Human (His)
Cat. No.:
HY-P701627
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