HCK Protein, Human (Sf9, His)

The HCK protein is a hematopoietic cell-dominated non-receptor tyrosine protein kinase that is critical for innate immune responses in neutrophils, monocytes, macrophages, and mast cells. HCK Protein, Human (Sf9, His) is the recombinant human-derived HCK protein, expressed by Sf9 insect cells , with N-8*His labeled tag. The total length of HCK Protein, Human (Sf9, His) is 446 a.a., .

HCK Protein, a non-receptor tyrosine-protein kinase predominantly found in hematopoietic cells, serves as a pivotal mediator transmitting signals from various cell surface receptors and playing a crucial role in the regulation of innate immune responses. It intricately modulates functions in neutrophils, monocytes, macrophages, and mast cells, influencing processes such as phagocytosis, cell survival, proliferation, adhesion, and migration. Acting downstream of receptors including FCGR1A, FCGR2A, CSF3R, PLAUR, IFNG, IL2, IL6, IL8, and integrins ITGB1 and ITGB2, HCK orchestrates critical events during the phagocytic process, including mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase, leading to the respiratory burst and release of inflammatory molecules. Additionally, HCK promotes the reorganization of the actin cytoskeleton, actin polymerization, formation of podosomes, and cell protrusions. It inhibits TP73-mediated transcription activation and apoptosis while phosphorylating key signaling proteins such as CBL, ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1, and WAS, highlighting its extensive involvement in diverse cellular pathways.

Human

Sf9 insect cells

N-8*His

P08631 (I81-P526, Q523E, Q524E, Q525I)

3055

Approximately 53.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.