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  3. hflC Protein, E.coli (His)

hflC Protein, E.coli (His)

Cat. No.: HY-P701912
Handling Instructions

The hflC protein cooperates with HflK to centrally regulate the stability of the phage lambda cII protein and affect the lysogenization frequency. HflC and HflK cooperate to form HflKC, which inhibits the SecY degradation activity of FtsH and may contribute to overall membrane protein quality control. hflC Protein, E.coli (His) is the recombinant E. coli-derived hflC protein, expressed by E. coli , with N-6*His labeled tag. The total length of hflC Protein, E.coli (His) is 334 a.a., .

For research use only. We do not sell to patients.

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Description

The hflC protein cooperates with HflK to centrally regulate the stability of the phage lambda cII protein and affect the lysogenization frequency. HflC and HflK cooperate to form HflKC, which inhibits the SecY degradation activity of FtsH and may contribute to overall membrane protein quality control. hflC Protein, E.coli (His) is the recombinant E. coli-derived hflC protein, expressed by E. coli , with N-6*His labeled tag. The total length of hflC Protein, E.coli (His) is 334 a.a., .

Background

The hflC Protein, in collaboration with HflK, plays a pivotal role in regulating the stability of the phage lambda cII protein, thereby exerting control over the lysogenization frequency of phage lambda. Beyond its involvement in phage dynamics, HflC, in conjunction with HflK, forms a complex known as HflKC, originally referred to as HflA. This complex interacts with FtsH, inhibiting its SecY-degrading activity and potentially contributing to the quality control of integral membrane proteins. The interaction between HflKC and FtsH is stimulated by ATP, emphasizing the dynamic nature of this regulatory mechanism. Additionally, HflKC engages with YccA, further expanding its network of interactions and underscoring its multifaceted role in cellular processes.

Species

E.coli

Source

E. coli

Tag

N-6*His

Accession

P0ABC4 (M1-R334)

Gene ID

75202409

Molecular Construction
N-term
6*His
hflC (M1-R334)
Accession # P0ABC4
C-term
Synonyms
hflC; Modulator of FtsH protease HflC
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

hflC Protein, E.coli (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
hflC Protein, E.coli (His)
Cat. No.:
HY-P701912
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