HGF Protein, Human (HEK293)

HGF Protein is a hepatocyte growth factor involved in signaling pathways including MET and MAPK.The HGF Protein consists of an alpha chain containing an n-terminal domain four kringle domains, and a β serine protease domain.HGF Protein, Human (HEK293) is the recombinant Virus-derived HGF protein, expressed by HEK293 , with tag free.

Hepatocyte growth factor (HGF) is a potent mitogen of liver cells that acts as a growth factor for a wide range of tissue and cell types. HGF is an activating ligand of Met receptor tyrosine kinase, whose activity is essential for normal cell development and organ regeneration, but abnormal activation of Met is associated with the growth, invasion, and metastasis of many types of solid tumors. HGF activates MAPK signals after TMPRSS13 cutting and activation. HGF is a double-stranded protein consisting of 728 amino acids, the α chain contains an n-terminal domain and four kringle domains, and the β chain contains a serine protease domain. HGF, a member of the plasminogen related growth factor family, is secreted as an inactive single-chain precursor that is proteolytic to form biologically active disulfide-linked α/β heterodimers^[1][2][3][4].

Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED₅₀ for this effect is 0.7597 ng/mL, corresponding to a specific activity is 1.316×10⁶ U/mg.

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  Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED₅₀ for this effect is 0.7597 ng/mL, corresponding to a specific activity is 1.316×10⁶ U/mg.

Human; Virus

HEK293

Tag Free

P14210-1 (Q32-S728)

3082

QRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGQPRPWCYTLDPHTRWEYCAIKTCADNTMNDTDVPLETTECIQGQGEGYRGTVNTIWNGIPCQRWDSQYPHEHDMTPENFKCKDLRENYCRNPDGSESPWCFTTDPNIRVGYCSQIPNCDMSHGQDCYRGNGKNYMGNLSQTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNENYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNIGWMVSLRYRNKHICGGSLIKESWVLTARQCFPSRDLKDYEAWLGIHDVHGRGDEKCKQVLNVSQLVYGPEGSDLVLMKLARPAVLDDFVSTIDLPNYGCTIPEKTSCSVYGWGYTGLINYDGLLRVAHLYIMGNEKCSQHHRGKVTLNESEICAGAEKIGSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKVPQS

32-36&52-65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.