ICAM-1/CD54 Protein, Rhesus Macaque (HEK293, Fc)

The ICAM-1/CD54 protein is a ligand of the leukocyte adhesion protein LFA-1 and is critical for leukocyte transendothelial migration. Its involvement activates ARHGEF26/SGEF and RHOG, coordinating endothelial apical cup assembly. ICAM-1/CD54 Protein, Rhesus Macaque (HEK293, Fc) is the recombinant Rhesus Macaque-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of ICAM-1/CD54 Protein, Rhesus Macaque (HEK293, Fc) is 453 a.a., with molecular weight of 100-130 kDa.

ICAM-1/CD54 Protein, as a ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2), plays a crucial role in leukocyte trans-endothelial migration. Engagement of ICAM-1 during this process promotes the assembly of endothelial apical cups through the activation of ARHGEF26/SGEF and RHOG, revealing its involvement in orchestrating dynamic cellular events (By similarity). ICAM-1 also forms homodimers and interacts with MUC1, fostering cell aggregation in epithelial cells. Furthermore, its interaction with ARHGEF26/SGEF underscores its participation in signaling pathways that regulate cellular dynamics. On the T cell side, ICAM-1 interacts with CD81, CD247, and CD9 at immunological synapses between antigen-presenting cells and T cells, emphasizing its pivotal role in immune cell interactions during the formation of immunological synapses. The multifaceted interactions of ICAM-1 highlight its significance in mediating diverse cellular processes and facilitating cell-cell communication.

Rhesus Macaque

HEK293

C-hFc

Q5NKV6 (Q28-E480)

712280  [NCBI]

QTSVFPPEVILPRGGSVKVNCSASCDQPISLGMETPLPKKEILPGGNNWKMYELSNVQEDSQPMCYSNCPDGQSSAKTLLTVYWTPERVELAPLPPWQPVGKNLTLRCQVEGGAPRANLTVMLLRGEKELSRQSAVGEPAEVTTTVPVGRDDHGANFSCRTELDLRPYVLKLFENTSAPHQLQTFDLPATPPQLVSPQVLEVDTQGTVVCSLDGLFPVSEAQVSLALGDQKLNPTITYGNNSLSAKASVKVTAEEEGTQQLLCGVMLGNQTQETRQTVTIYSFPAPNVNLTKPEVSEGTEVIVECEAHPRAKVMLNGVPAQPPGPRAQFLLKATPEDNGRSFSCSATLEVAGQLVHKNQTRELRVLYGPRLDEKDCPGNWTWPENSQQTPMCQAWGNPLPQLKCLKDGTFPLPIGQSVTVTRDLEGTYLCQARSTRGEVTREVTVNVLSPRYE

100-130 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.