IGFBP-4 Protein, Cynomolgus (HEK293, His)

IGFBP-4 protein lacks essential conserved residue(s) for propagating feature annotation. IGFBP-4 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IGFBP-4 protein, expressed by HEK293 , with C-His labeled tag. The total length of IGFBP-4 Protein, Cynomolgus (HEK293, His) is 235 a.a., with molecular weight of 33-36 kDa.

IGFBP-4 Protein lacks conserved residue(s) required for the propagation of feature annotation.

Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells. The ED₅₀ for this effect is typically 0.04-0.2 μg/mL in the presence of 14 ng/mL human IGFII.

Cynomolgus

HEK293

C-His

XP_005584157.1 (D22-E258)

NC_088390.1

33-36 kDa

Greater than 80% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.