IL-2 Protein, Rat (145a.a, HEK293, C-His)

IL-2 Protein, produced by activated T-cells, NK cells, and helper T-cells, is crucial for immune response and tolerance. It binds to receptor complexes, activating downstream signaling pathways. IL-2 Protein acts as a growth factor, enhances NK-cell activity, promotes B-cell proliferation, and regulates regulatory T-cells. It also participates in differentiating effector T-cell subsets and memory CD8-positive T-cells. IL-2 Protein, Rat (145a.a, HEK293, C-His) is the recombinant rat-derived IL-2 protein, expressed by HEK293 , with C-10*His labeled tag.

IL-2, a cytokine primarily produced by activated CD4-positive helper T-cells, and to a lesser extent by activated CD8-positive T-cells and natural killer (NK) cells, plays pivotal roles in immune response and tolerance. It binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122, and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). The interaction triggers oligomerization and conformational changes in the IL-2R subunits, initiating downstream signaling that begins with the phosphorylation of JAK1 and JAK3. Consequently, JAK1 and JAK3 phosphorylate the receptor, creating a docking site for the phosphorylation of substrates, including STAT5, activating pathways such as STAT, phosphoinositide-3-kinase/PI3K, and mitogen-activated protein kinase/MAPK. IL-2 functions as a T-cell growth factor, enhances NK-cell cytolytic activity, and fosters robust proliferation of activated B-cells, leading to increased immunoglobulin production. Crucially, IL-2 plays a pivotal role in regulating the adaptive immune system by governing the survival and proliferation of regulatory T-cells, crucial for immune tolerance maintenance. Furthermore, it participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17, as well as memory CD8-positive T-cells.

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED₅₀ this effect is 0.1884 ng/mL, corresponding to a specific activity is 5.307×10⁶ units/mg.

• 
  Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED₅₀ this effect is 0.1884 ng/mL, corresponding to a specific activity is 5.307×10⁶ units/mg.

Rat

HEK293

C-10*His

P17108 (A11-Q155)

116562  [NCBI]

ALTLVLLVNSAPTSSPAKETQQHLEQLLLDLQVLLRGIDNYKNLKLPMMLTFKFYLPKQATELKHLQCLENELGALQRVLDLTQSKSFHLEDAGNFISNIRVTVVKLKGSENKFECQFDDEPATVVEFLRRWIAICQSIISTMTQ

Approximately 18 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.