1. Recombinant Proteins
  2. Cytokines and Growth Factors Receptor Proteins
  3. Interleukin & Receptors Cytokine Receptors
  4. IL-22 Receptor
  5. IL-22R alpha 1
  6. IL-22R alpha 1 Protein, Cynomolgus (HEK293, His)

IL-22R alpha 1 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P700744
Data Sheet Handling Instructions Technical Support

IL-22R α 1 and IL-10R β together form IL20, IL22 and IL24 receptor complexes, activating different signaling pathways. Within the IL22 receptor, IL-22R α 1 binds to IL10RB and mediates IL22 signaling through the JAK/STAT and MAPK1/MAPK3/Akt pathways. IL-22R alpha 1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IL-22R alpha 1 protein, expressed by HEK293 , with C-His labeled tag. The total length of IL-22R alpha 1 Protein, Cynomolgus (HEK293, His) is 208 a.a., with molecular weight of 38-48 kDa.

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Size Price Stock Quantity
20 μg USD 160 In-stock
50 μg USD 300 In-stock
100 μg USD 480 In-stock
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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

IL-22R α 1 and IL-10R β together form IL20, IL22 and IL24 receptor complexes, activating different signaling pathways. Within the IL22 receptor, IL-22R α 1 binds to IL10RB and mediates IL22 signaling through the JAK/STAT and MAPK1/MAPK3/Akt pathways. IL-22R alpha 1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived IL-22R alpha 1 protein, expressed by HEK293 , with C-His labeled tag. The total length of IL-22R alpha 1 Protein, Cynomolgus (HEK293, His) is 208 a.a., with molecular weight of 38-48 kDa.

Background

IL-22R alpha 1, a key component of the receptor for IL20, IL22, and IL24, plays a crucial role in mediating the signaling pathways activated by these interleukins. It forms a heterodimer with IL10RB, constituting the IL22 receptor and facilitating IL22 signaling via JAK/STAT pathways. IL-22R alpha 1 is also part of another receptor complex, along with IL20RB, responding to IL20 and IL24, leading to the activation of STATs. Beyond JAK/STAT pathways, IL-22 induces MAPK1/MAPK3 and Akt kinases activation. Notably, IL-22R alpha 1 contributes to the antiangiogenic activity of IL24 and inhibits endothelial cell tube formation and differentiation induced by IL24. In its functional heterodimeric associations with IL10RB and IL20RB, IL-22R alpha 1 underscores its pivotal role in mediating the diverse cellular responses to IL20, IL22, and IL24.

Biological Activity

Cynomolgus IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-22, His Tag with an affinity constant of 58-61 nM as determined in SPR assay (Biacore T200).

Species

Cynomolgus

Source

HEK293

Tag

C-His

Accession

A0A2K5TKX9 (P18-T225)

Gene ID

102117911

Molecular Construction
N-term
IL-22Rα 1 (P18-T225)
Accession # A0A2K5TKX9
His
C-term
Synonyms
IL-22R-alpha-1; IL-22RA1; CRF2-9; IL22R; IL22R1; IL-TIF-R1; zcytoR11
Molecular Weight

38-48 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IL-22R alpha 1 Protein, Cynomolgus (HEK293, His) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-22R alpha 1 Protein, Cynomolgus (HEK293, His)
Cat. No.:
HY-P700744
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