IL-4R alpha protein, Canine (HEK293, His)

IL-4R alpha protein, Canine (HEK293, His) is the recombinant canine-derived IL-4R alpha protein, expressed by HEK293 , with C-6*His labeled tag. The total length of IL-4R alpha protein, Canine (HEK293, His) is 229 a.a., with molecular weight of 35-55 kDa.

Measured by its ability to inhibit IL-4-dependent proliferation of TF 1 human erythroleukemic cells. The ED₅₀ for this effect is 18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL-4, corresponding to a specific activity is 5.482×10⁴ units/mg.

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  Measured by its ability to inhibit IL-4-dependent proliferation of TF 1 human erythroleukemic cells. The ED₅₀ for this effect is 18.24 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL‑4, corresponding to a specific activity is 5.482×10⁴ units/mg.

Canine

HEK293

C-6*His

XP_022275636.1 (G2-Q230)

489957  [NCBI]

GRLCSGLTFPVSCLVLVWVASSGSVKVLHEPSCFSDYISTSVCQWKMDHPTNCSAELRLSYQLDFMGSENHTCVPENREDSVCVCSMPIDDAVEADVYQLDLWAGQQLLWSGSFQPSKHVKPRTPGNLTVHPNISHTWLLMWTNPYPTENHLHSELTYMVNVSNDNDPEDFKVYNVTYMGPTLRLAASTLKSGASYSARVRAWAQTYNSTWSDWSPSTTWLNYYEPWEQ

Approximately 45 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.