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  5. IL-4R alpha/CD124
  6. IL-4R Protein, Cynomolgus (HEK293, His)

IL-4R Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P77010
Data Sheet Handling Instructions

IL-4R alpha is a subunit alpha shared by IL-4 and IL-13 receptors, found in leukocytes originally. IL-4R alpha couples to the JAK1/2/3-STAT6 pathway and involves in promoting Th2 differentiation. IL-4R alpha/CD124, Cynomolgus consists of 825 amino acids (M1-S825). IL-4R alpha/CD124 Protein, Cynomolgus is produced in HEK293 cells, including a Fibronectin typr III domain (125-224 a.a) and a C-Terminal His-tag.

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Description

IL-4R alpha is a subunit alpha shared by IL-4 and IL-13 receptors, found in leukocytes originally. IL-4R alpha couples to the JAK1/2/3-STAT6 pathway and involves in promoting Th2 differentiation[1]. IL-4R alpha/CD124, Cynomolgus consists of 825 amino acids (M1-S825). IL-4R alpha/CD124 Protein, Cynomolgus is produced in HEK293 cells, including a Fibronectin typr III domain (125-224 a.a) and a C-Terminal His-tag.

Background

Interleukin-4R alpha (IL-4Rα), also known as CD124 and B cell stimulatory factor (BSF) receptor, is one of the anti-inflammatory cytokines, and highly expressed in activated T-cells[1].
IL-4R alpha participates in forming two interleukin receptors in different cell types. For the type I receptor, depends on IL-4R alpha binding IL-4 to recruit IL-2R gamma chain in immune cells. IL-2R gamma is the common subunit for a variety of interleukin receptors, involved in the stimulation of neutrophil phagocytosis by IL-15. For the type II receptor, depends on IL-4R alpha binding IL-4 to recruit IL-13R alpha 1 chain. IL-13R alpha 1 is an alternat accessory protein to the common cytokine receptor gamma chain in non-immune cells[2][3].
The sequence of amino acids in IL-4R alpha proteins is very different among different animals.
IL-4 R alpha generates a soluble form by alternate splicing or proteolysis, maintaining ligand binding properties and inhibiting IL-4 bioactivity. IL-4 R alpha soluble isoform 1 can be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase[4].
IL-4 R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis[5].

In Vitro

Interleukin-4Rα (IL-4Rα) shows promotion of Th2 cytokine and IgE response without hIL-4, and fails to expel N. brasiliensis worms in transgenic mice (hIL-4RαTg/mIL-4Rα-/-) infected with Nippostrongylus brasiliensis[8].

In Vivo

IL-4Rα is regulated by variant IL-4 (VIL-4), produced by Mycobacterium tuberculosis and bacillus Calmette-Guérin infections of macaques, and induces expansion of Vgamma2Vdelta2 T cells[6]

Species

Cynomolgus

Source

HEK293

Tag

C-His

Accession

G7Q0S7/EHH60265.1 (M26-R232)

Gene ID

/

Molecular Construction
N-term
IL-4R (M26-R232)
Accession # G7Q0S7/EHH60265.1
His
C-term
Synonyms
Interleukin-4 receptor subunit alpha; IL4R alpha; IL4R; CD124
Molecular Weight

Approximately 25.2 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
References

IL-4R Protein, Cynomolgus (HEK293, His) Related Classifications

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-4R Protein, Cynomolgus (HEK293, His)
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