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  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. JMJD2D Protein, Human

JMJD2D Protein, Human

Cat. No.: HY-P701620
Handling Instructions

The JMJD2D protein is a core histone demethylase in the complex histone code, specifically targeting "Lys-9" of histone H3. Importantly, it does not demethylate H3 "Lys-4", "Lys-27", "Lys-36" or H4 "Lys-20". JMJD2D Protein, Human is the recombinant human-derived JMJD2D protein, expressed by E. coli , with tag free. The total length of JMJD2D Protein, Human is 522 a.a., .

For research use only. We do not sell to patients.

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Description

The JMJD2D protein is a core histone demethylase in the complex histone code, specifically targeting "Lys-9" of histone H3. Importantly, it does not demethylate H3 "Lys-4", "Lys-27", "Lys-36" or H4 "Lys-20". JMJD2D Protein, Human is the recombinant human-derived JMJD2D protein, expressed by E. coli , with tag free. The total length of JMJD2D Protein, Human is 522 a.a., .
Background

JMJD2D Protein, a histone demethylase, assumes a central role in the intricate histone code by specifically targeting 'Lys-9' of histone H3. Notably, JMJD2D does not demethylate histone H3 'Lys-4,' H3 'Lys-27,' H3 'Lys-36,' nor H4 'Lys-20.' Its demethylase activity is confined to both di- and trimethylated H3 'Lys-9' residues, with no discernible effect on monomethylated residues. The demethylation process catalyzed by JMJD2D leads to the generation of formaldehyde and succinate. This specific demethylation pattern underscores JMJD2D's significance in shaping the epigenetic landscape, shedding light on its distinct role in the dynamic regulation of histone modifications.
Species

Human
Source

E. coli
Tag

Tag Free
Accession

Q6B0I6 (E2-P523)
Gene ID

55693
Molecular Construction
N-term
JMJD2D (E2-P523)
Accession # Q6B0I6
C-term
Synonyms
KDM4D; Lysine-specific demethylase 4D; JmjC domain-containing histone demethylation protein 3D; Jumonji domain-containing protein 2D; [histone H3]-trimethyl-L-lysine(9) demethylase 4D
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

JMJD2D Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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