Kallikrein-4 Protein, Human (H197Q, HEK293, His)

Kallikrein-4 Protein is crucial in enamel formation, particularly during tooth maturation, playing a significant role in clearing enamel proteins and contributing to the structural patterning of the crystalline matrix. Its involvement underscores its importance in intricate tooth development processes, emphasizing its role in proper dental structure and function. Kallikrein-4 Protein, Human (H197Q, HEK293, His) is the recombinant human-derived Kallikrein-4 protein, expressed by HEK293 , with C-6*His labeled tag and H197Q, , , , mutation. The total length of Kallikrein-4 Protein, Human (H197Q, HEK293, His) is 228 a.a., with molecular weight of 25-32 kDa.

Kallikrein-4 Protein plays a significant role in enamel formation, particularly during the maturation stage of tooth development. It is essential for the clearance of enamel proteins and contributes to the normal structural patterning of the crystalline matrix. This underscores the protein's crucial involvement in the intricate processes underlying tooth development and enamel maturation, highlighting its importance for proper dental structure and function.

Measured by its ability to cleave 100μM fluorogenic peptide substrate Boc-VPR-AMC (HY-137784) that incubate at 37℃ in kinetic mode for 5 minutes. The specific activity is 412.94 pmol/min/µg.

Human

HEK293

C-6*His

Q9Y5K2 (S27-S254, H197Q)

9622  [NCBI]

SCSQIINGEDCSPHSQPWQAALVMENELFCSGVLVHPQWVLSAAHCFQNSYTIGLGLHSLEADQEPGSQMVEASLSVRHPEYNRPLLANDLMLIKLDESVSESDTIRSISIASQCPTAGNSCLVSGWGLLANGRMPTVLQCVNVSVVSEEVCSKLYDPLYHPSMFCAGGGQDQKDSCNGDSGGPLICNGYLQGLVSFGKAPCGQVGVPGVYTNLCKFTEWIEKTVQAS

25-32 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.