2. Recombinant Proteins
  3. Cytokines and Growth Factors
  4. Laminin 521 Protein, Human

Laminin 521 Protein, Human

Cat. No.: HY-P701311
COA Handling Instructions

Laminins are a major component of basement membranes. Laminin 521 is a heterotrimeric glycoprotein composed of one α5, one β2, and one γ1 chain, is a defined matrix for feeder-free culture of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). When used in combination with defined maintenance media, Laminin 521 reliably facilitates self-renewal of human ESCs and iPSCs without spontaneous differentiation.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
20 μg $40 In-stock
50 μg $75 In-stock
100 μg $120 In-stock
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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

Laminins are a major component of basement membranes. Laminin 521 is a heterotrimeric glycoprotein composed of one α5, one β2, and one γ1 chain, is a defined matrix for feeder-free culture of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). When used in combination with defined maintenance media, Laminin 521 reliably facilitates self-renewal of human ESCs and iPSCs without spontaneous differentiation.
Species

Human
Source

/
Accession

/
Gene ID

/
Synonyms
Laminin 521
Molecular Weight

/
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Solution
Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin Level

<1 EU/mL, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation

COA (204 KB)

Handling Instructions (2659 KB)

Laminin 521 Protein, Human Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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