Leptin R/LEPR Protein, Human (HEK293, His)

Leptin R/LEPR protein, as the receptor of LEP/leptin, plays a key role in mediating central and peripheral effects through multiple signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, it regulates appetite, influencing orexigenic factors and inhibiting orexigenic neuropeptides. Leptin R/LEPR Protein, Human (HEK293, His) is the recombinant human-derived Leptin R/LEPR protein, expressed by HEK293 , with C-10*His labeled tag.

Leptin R/LEPR Protein functions as a receptor for the hormone LEP/leptin, playing a pivotal role in mediating its central and peripheral effects through the activation of diverse signaling pathways, including JAK2/STAT3 and the MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor, inducing a decrease in food intake and an increase in energy consumption by influencing anorexinogenic factors and suppressing orexigenic neuropeptides. Furthermore, it regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, Leptin R/LEPR impacts basal metabolism, reproductive function, pancreatic beta-cell function, insulin secretion, angiogenesis, and innate and adaptive immunity. The control of energy homeostasis and melanocortin production, as well as the regulation of fertility, growth, and glucose homeostasis, involves distinct signaling pathways. Additionally, Leptin R/LEPR participates in the counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus and has specific effects on T lymphocyte responses, modulating the proliferation of naive and memory T cells and influencing cytokine production. Moreover, it may serve as a transporter for LEP across the blood-brain barrier and is involved in LEP endocytosis, albeit without inducing phosphorylation of and activating STAT3.

Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 5 μg/mL (100 μL/well) can bind human Leptin receptor. The ED₅₀ for this effect is 0.2725 μg/mL.

Human

HEK293

C-10*His

P48357-1 (F22-D839)

3953  [NCBI]

FNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHRYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINSIGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSD

Approximately 100-180 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.