1. Recombinant Proteins
  2. Receptor Proteins
  3. Leptin R/LEPR Protein, Human (HEK293, His)

Leptin R/LEPR protein, as the receptor of LEP/leptin, plays a key role in mediating central and peripheral effects through multiple signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, it regulates appetite, influencing orexigenic factors and inhibiting orexigenic neuropeptides. Leptin R/LEPR Protein, Human (HEK293, His) is the recombinant human-derived Leptin R/LEPR protein, expressed by HEK293 , with C-10*His labeled tag.

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  • Biological Activity

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Description

Leptin R/LEPR protein, as the receptor of LEP/leptin, plays a key role in mediating central and peripheral effects through multiple signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, it regulates appetite, influencing orexigenic factors and inhibiting orexigenic neuropeptides. Leptin R/LEPR Protein, Human (HEK293, His) is the recombinant human-derived Leptin R/LEPR protein, expressed by HEK293 , with C-10*His labeled tag.

Background

Leptin R/LEPR Protein functions as a receptor for the hormone LEP/leptin, playing a pivotal role in mediating its central and peripheral effects through the activation of diverse signaling pathways, including JAK2/STAT3 and the MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor, inducing a decrease in food intake and an increase in energy consumption by influencing anorexinogenic factors and suppressing orexigenic neuropeptides. Furthermore, it regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, Leptin R/LEPR impacts basal metabolism, reproductive function, pancreatic beta-cell function, insulin secretion, angiogenesis, and innate and adaptive immunity. The control of energy homeostasis and melanocortin production, as well as the regulation of fertility, growth, and glucose homeostasis, involves distinct signaling pathways. Additionally, Leptin R/LEPR participates in the counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus and has specific effects on T lymphocyte responses, modulating the proliferation of naive and memory T cells and influencing cytokine production. Moreover, it may serve as a transporter for LEP across the blood-brain barrier and is involved in LEP endocytosis, albeit without inducing phosphorylation of and activating STAT3.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 5 μg/mL (100 μL/well) can bind human Leptin receptor. The ED50 for this effect is 0.2725 μg/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 5 μg/mL (100 μL/well) can bind human Leptin receptor. The ED50 for this effect is 0.2725 μg/mL.
Species

Human

Source

HEK293

Tag

C-10*His

Accession

P48357-1 (F22-D839)

Gene ID
Molecular Construction
N-term
LEPR (F22-D839)
Accession # P48357-1
10*His
C-term
Synonyms
Leptin receptor; LEP-R; HuB219; OB receptor; OB-R; CD295; LEPR; DB; OBR
AA Sequence

FNLSYPITPWRFKLSCMPPNSTYDYFLLPAGLSKNTSNSNGHYETAVEPKFNSSGTHFSNLSKTTFHCCFRSEQDRNCSLCADNIEGKTFVSTVNSLVFQQIDANWNIQCWLKGDLKLFICYVESLFKNLFRNYNYKVHLLYVLPEVLEDSPLVPQKGSFQMVHCNCSVHECCECLVPVPTAKLNDTLLMCLKITSGGVIFQSPLMSVQPINMVKPDPPLGLHMEITDDGNLKISWSSPPLVPFPLQYQVKYSENSTTVIREADKIVSATSLLVDSILPGSSYEVQVRGKRLDGPGIWSDWSTPRVFTTQDVIYFPPKILTSVGSNVSFHCIYKKENKIVPSKEIVWWMNLAEKIPQSQYDVVSDHVSKVTFFNLNETKPRGKFTYDAVYCCNEHECHHRYAELYVIDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVVMDIKVPMRGPEFWRIINGDTMKKEKNVTLLWKPLMKNDSLCSVQRYVINHHTSCNGTWSEDVGNHTKFTFLWTEQAHTVTVLAINSIGASVANFNLTFSWPMSKVNIVQSLSAYPLNSSCVIVSWILSPSDYKLMYFIIEWKNLNEDGEIKWLRISSSVKKYYIHDHFIPIEKYQFSLYPIFMEGVGKPKIINSFTQDDIEKHQSD

Molecular Weight

Approximately 100-180 kDa due to the glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 8.0, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Leptin R/LEPR Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Inquiry Information

Product Name:
Leptin R/LEPR Protein, Human (HEK293, His)
Cat. No.:
HY-P72524
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