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  4. LGP2 Protein, Human (HEK293, FLAG)

LGP2 Protein, Human (HEK293, FLAG)

Cat. No.: HY-P701998
Handling Instructions

LGP2 protein is a key regulator of RIGI- and IFIH1/MDA5-mediated antiviral signaling and has dual negative and positive functions affected by factors such as infected viruses and target cells. LGP2 lacks a CARD domain for independent initiation of antiviral signaling and therefore inhibits RIG-I through multiple mechanisms, including competition for RNA binding and obstruction of interaction with MAVS/IPS1. LGP2 Protein, Human (HEK293, FLAG) is the recombinant human-derived LGP2 protein, expressed by HEK293 , with C-Flag labeled tag. The total length of LGP2 Protein, Human (HEK293, FLAG) is 678 a.a., .

For research use only. We do not sell to patients.

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Description

LGP2 protein is a key regulator of RIGI- and IFIH1/MDA5-mediated antiviral signaling and has dual negative and positive functions affected by factors such as infected viruses and target cells. LGP2 lacks a CARD domain for independent initiation of antiviral signaling and therefore inhibits RIG-I through multiple mechanisms, including competition for RNA binding and obstruction of interaction with MAVS/IPS1. LGP2 Protein, Human (HEK293, FLAG) is the recombinant human-derived LGP2 protein, expressed by HEK293 , with C-Flag labeled tag. The total length of LGP2 Protein, Human (HEK293, FLAG) is 678 a.a., .

Background

LGP2 Protein functions as a key regulator of RIGI and IFIH1/MDA5-mediated antiviral signaling, contributing both negative and positive regulatory functions that are contingent on various factors such as the infecting virus, target cells, or a combination of both. Lacking the CARD domain necessary for activating MAVS/IPS1-dependent signaling events, LGP2 cannot initiate antiviral signaling on its own. Its inhibitory effects on RIG-I signaling may involve multiple mechanisms, including competition with RIGI for binding to viral RNA, direct binding to RIGI to impede dimerization and interaction with MAVS/IPS1, and competition with IKBKE for binding to MAVS/IPS1, thereby hindering IRF3 activation. Conversely, its positive regulatory role may encompass activities such as unwinding or stripping nucleoproteins from viral RNA, thereby facilitating recognition by RIGI and IFIH1/MDA5. LGP2 plays a crucial role in the innate immune response to various RNA viruses, DNA viruses like poxviruses and coronavirus SARS-CoV-2, as well as the bacterial pathogen Listeria monocytogenes, highlighting its versatile involvement in antiviral defense. The protein exhibits affinity for both single-stranded and double-stranded RNA, with a preference for 5'-triphosphorylated RNA, although it can recognize RNA lacking this modification.

Species

Human

Source

HEK293

Tag

C-Flag

Accession

Q96C10 (M1-D678)

Gene ID

79132

Molecular Construction
N-term
LGP2 (M1-D678)
Accession # Q96C10
Flag
C-term
Synonyms
CGAS; Probable ATP-dependent RNA helicase DHX58; Probable ATP-dependent helicase LGP2; Protein D11Lgp2 homolog; RIG-I-like receptor 3; RLR-3; RIG-I-like receptor LGP2; RLR
Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

LGP2 Protein, Human (HEK293, FLAG) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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LGP2 Protein, Human (HEK293, FLAG)
Cat. No.:
HY-P701998
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