1. Recombinant Proteins
  2. Others
  3. Lgr5/GPR49 Protein, Human (CHO, hFc)

The Lgr5/GPR49 protein is a receptor for R-spondins that enhances the canonical Wnt signaling pathway and marks stem cells in the intestinal epithelium and hair follicles. Lgr5/GPR49 binds R-spondins (RSPO1-4), binds to phosphorylated LRP6 and Frizzled receptors, initiates Wnt signaling and amplifies target gene expression. Lgr5/GPR49 Protein, Human (CHO, hFc) is the recombinant human-derived Lgr5/GPR49 protein, expressed by CHO , with C-hFc labeled tag. The total length of Lgr5/GPR49 Protein, Human (CHO, hFc) is 539 a.a., with molecular weight of 88-110 kDa.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
5 μg USD 43 In-stock
10 μg USD 74 In-stock
50 μg USD 206 Get quote
100 μg   Get quote  

Get it by April 10 for select sizes. Order within 18 hrs 39 mins.

* Please select Quantity before adding items.

Top Publications Citing Use of Products

Publications Citing Use of MCE Lgr5/GPR49 Protein, Human (CHO, hFc)

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The Lgr5/GPR49 protein is a receptor for R-spondins that enhances the canonical Wnt signaling pathway and marks stem cells in the intestinal epithelium and hair follicles. Lgr5/GPR49 binds R-spondins (RSPO1-4), binds to phosphorylated LRP6 and Frizzled receptors, initiates Wnt signaling and amplifies target gene expression. Lgr5/GPR49 Protein, Human (CHO, hFc) is the recombinant human-derived Lgr5/GPR49 protein, expressed by CHO , with C-hFc labeled tag. The total length of Lgr5/GPR49 Protein, Human (CHO, hFc) is 539 a.a., with molecular weight of 88-110 kDa.

Background

The Lgr5/GPR49 Protein serves as the receptor for R-spondins, functioning to potentiate the canonical Wnt signaling pathway while concurrently serving as a distinctive stem cell marker in the intestinal epithelium and hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3, or RSPO4), Lgr5/GPR49 associates with phosphorylated LRP6 and frizzled receptors activated by extracellular Wnt, initiating the canonical Wnt signaling pathway and amplifying the expression of target genes. In contrast to classical G-protein coupled receptors, Lgr5/GPR49 does not activate heterotrimeric G-proteins to transduce the signal, highlighting its unique regulatory role in Wnt signaling. It plays a crucial role in the development and maintenance of adult intestinal stem cells during postembryonic development and has been identified in a complex with RNF43 and RSPO1. Additionally, Lgr5/GPR49 interacts with other R-spondin ligands, including RSPO2, RSPO3, and RSPO4, emphasizing its diverse participation in cellular signaling pathways.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Human R-Spondin 1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human LGR5. The ED50 for this effect is 42.14 ng/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized Human R-Spondin 1 at 5 μg/mL (100 μL/well) can bind Biotinylated Human LGR5.The ED50 for this effect is 42.14 ng/mL.
Species

Human

Source

CHO

Tag

C-hFc

Accession

O75473 (G22-I560)

Gene ID
Molecular Construction
N-term
Lgr5 (G22-I560)
Accession # O75473
hFc
C-term
Synonyms
Leucine-rich repeat-containing G-protein coupled receptor 5; LGR5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38; GPR49; GPR67; Leucine-rich Repeat Containing G Protein-coupled Receptor 5
AA Sequence

GSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLI

Molecular Weight

88-110 kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Lgr5/GPR49 Protein, Human (CHO, hFc) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Lgr5/GPR49 Protein, Human (CHO, hFc)
Cat. No.:
HY-P79308
Quantity:
MCE Japan Authorized Agent: