LIMS1 Protein, Mouse (Baculovirus, His)

LIMS1, an adapter protein, crucially links beta-integrins to the actin cytoskeleton and cell surface receptors. Mediating vital cellular processes, it connects with integrin-linked protein kinase 1 (ILK) through its first LIM domain, competing with LIMS2. LIMS1 contributes to the IPP complex, with ILK and LIMS1/LIMS2, vital in cellular signaling. Additionally, it binds to NCK2, linking the complex to cell surface receptors, and interacts with TGFB1I1 through the LIM zinc-binding 5 domain, expanding its molecular associations. LIMS1 Protein, Mouse (Baculovirus, His) is the recombinant mouse-derived LIMS1 protein, expressed by Sf9 insect cells, with N-10*His labeled tag. The total length of LIMS1 Protein, Mouse (Baculovirus, His) is 324 a.a., with molecular weight of ~39.6 kDa.

LIMS1, functioning as an adapter protein within a cytoplasmic complex, serves as a crucial link between beta-integrins and the actin cytoskeleton, while simultaneously connecting the complex to cell surface receptor tyrosine kinases and growth factor receptors. Its involvement spans the regulation of essential cellular processes, including cell survival, proliferation, and differentiation. Through the first LIM domain, LIMS1 interacts with integrin-linked protein kinase 1 (ILK), engaging in competitive binding with LIMS2. Notably, LIMS1 contributes to the heterotrimeric IPP complex, alongside ILK and either LIMS1 or LIMS2, which plays a pivotal role in cellular signaling. Furthermore, LIMS1 establishes connections with the SH3/SH2 adapter NCK2, facilitating the linkage of the complex to cell surface receptors. Its interaction with TGFB1I1, mediated by the LIM zinc-binding 5 domain, further expands its network of molecular associations.

Mouse

Sf9 insect cells

N-10*His

Q99JW4 (A2-K325)

110829  [NCBI]

ANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFIIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGDVVSALNKAWCVSCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLSETLGRK

Approximately 39.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.