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Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO)

Cat. No.: HY-P71524
Handling Instructions Technical Support

Lipoic acid protein ligase A (LplA) is a key enzyme involved in the activation and transfer of lipoic acid, an important cofactor, to the lipoyl domain of lipoic acid-dependent enzymes. LplA catalyzes the ATP-dependent activation of exogenous lipoic acid, converting it to lipoyl-AMP as an intermediate. Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Lipoate-protein ligase A/LplA protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO) is 338 a.a., with molecular weight of ~53.9 kDa.

For research use only. We do not sell to patients.

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Description

Lipoic acid protein ligase A (LplA) is a key enzyme involved in the activation and transfer of lipoic acid, an important cofactor, to the lipoyl domain of lipoic acid-dependent enzymes. LplA catalyzes the ATP-dependent activation of exogenous lipoic acid, converting it to lipoyl-AMP as an intermediate. Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Lipoate-protein ligase A/LplA protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO) is 338 a.a., with molecular weight of ~53.9 kDa.

Background

Lipoate-protein ligase A (LplA) is a crucial enzyme involved in the activation and transfer of lipoate, a vital cofactor, onto the lipoyl domains of lipoate-dependent enzymes. LplA catalyzes the ATP-dependent activation of exogenously supplied lipoate, converting it into lipoyl-AMP, which serves as an intermediate. This activated lipoyl-AMP is then utilized by LplA to transfer the lipoyl moiety onto target proteins. By facilitating this transfer process, LplA ensures the proper functioning of lipoate-dependent enzymes, which are involved in various essential metabolic pathways such as the citric acid cycle and the metabolism of fatty acids. Understanding the mechanisms and regulation of LplA-mediated lipoate activation and transfer can provide insights into cellular energy metabolism and the maintenance of metabolic homeostasis.

Species

E.coli

Source

E. coli

Tag

N-SUMO;N-6*His

Accession

C4ZT68 (M1-R338)

Gene ID

/

Molecular Construction
N-term
6*His-SUMO
LplA (M1-R338)
Accession # C4ZT68
C-term
Synonyms
lplA; BWG_4078Lipoate-protein ligase A; EC 6.3.1.20; Lipoate--protein ligase
AA Sequence

MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTISTSIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEAEIISPNKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMAGAVR

Molecular Weight

Approximately 53.9 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Lipoate-protein ligase A/LplA Protein, E.coli (His-SUMO)
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HY-P71524
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