LRP-6 Protein, Human (HEK293, mFc)

LRP-6 protein is a key component of the Wnt-Fzd-LRP5-LRP6 complex, a component of β-catenin signaling, and is essential for bone formation. LRP-6 acts as a cell surface co-receptor and coordinates the formation of signalosomes by clustering receptor-ligand complexes. LRP-6 Protein, Human (HEK293, mFc) is the recombinant human-derived LRP-6 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of LRP-6 Protein, Human (HEK293, mFc) is 611 a.a., with molecular weight of 100-120 kDa.

As an integral component of the Wnt-Fzd-LRP5-LRP6 complex, LRP-6 protein plays a crucial role in initiating beta-catenin signaling by orchestrating the aggregation of receptor-ligand complexes into ribosome-sized signalosomes. Functioning as a cell-surface coreceptor in Wnt/beta-catenin signaling, it is particularly pivotal in bone formation. The complex formed by Wnt-induced Fzd/LRP6 coreceptor recruits DVL1 polymers to the plasma membrane, facilitating the recruitment of the AXIN1/GSK3B complex to the cell surface. This, in turn, promotes the formation of signalosomes, inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. LRP-6 is indispensable for the posterior patterning of the epiblast during gastrulation. The protein engages in a myriad of interactions, including those with various WNT proteins, FZD5, DKK1, DRAXIN, AXIN1, GRB10, RSPO1, RSPO3, SOST, MESD, CSNKIE, MACF1, DAB2, TMEM198, CAPRIN2, LYPD6, KREM1, and MDK, highlighting its versatility and regulatory roles in the Wnt signaling pathway.

1.Immobilized Human LRP-6, mFc Tag at 5 μg/mL (100 μl/Well) on plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC₅₀ of <2.1 μg/mL determined by ELISA.
2.Immobilized Biotinylated Human SOST, His Tag at 5 μg/mL (100μl/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human LRP-6, mFc Tag with the EC₅₀ of ≤0.17 μg/mL determined by ELISA.

Human

HEK293

C-mFc

O75581 (A20-P630)

4040  [NCBI]

APLLLYANRRDLRLVDATNGKENATIVVGGLEDAAAVDFVFSHGLIYWSDVSEEAIKRTEFNKTESVQNVVVSGLLSPDGLACDWLGEKLYWTDSETNRIEVSNLDGSLRKVLFWQELDQPRAIALDPSSGFMYWTDWGEVPKIERAGMDGSSRFIIINSEIYWPNGLTLDYEEQKLYWADAKLNFIHKSNLDGTNRQAVVKGSLPHPFALTLFEDILYWTDWSTHSILACNKYTGEGLREIHSDIFSPMDIHAFSQQRQPNATNPCGIDNGGCSHLCLMSPVKPFYQCACPTGVKLLENGKTCKDGATELLLLARRTDLRRISLDTPDFTDIVLQLEDIRHAIAIDYDPVEGYIYWTDDEVRAIRRSFIDGSGSQFVVTAQIAHPDGIAVDWVARNLYWTDTGTDRIEVTRLNGTMRKILISEDLEEPRAIVLDPMVGYMYWTDWGEIPKIERAALDGSDRVVLVNTSLGWPNGLALDYDEGKIYWGDAKTDKIEVMNTDGTGRRVLVEDKIPHIFGFTLLGDYVYWTDWQRRSIERVHKRSAEREVIIDQLPDLMGLKATNVHRVIGSNPCAEENGGCSHLCLYRPQGLRCACPIGFELISDMKTCIVP

100-120 kDa

Greater than 90% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.