MCR-1 protein, E.coli (364a.a, His-SUMO)

The MCR-1 protein may catalyze the addition of a phosphoethanolamine moiety to lipid A, which is associated with polymyxin resistance. This modification observed in E. coli expressing MCR-1 increased the minimum inhibitory concentration (MIC) of colistin and polymyxin B. MCR-1 protein, E.coli (364a.a, His-SUMO) is the recombinant E. coli-derived MCR-1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of MCR-1 protein, E.coli (364a.a, His-SUMO) is 364 a.a., with molecular weight of ~56.7 kDa.

The MCR-1 protein is likely responsible for catalyzing the addition of a phosphoethanolamine moiety to lipid A, a modification associated with polymyxin resistance. This modification plays a crucial role in conferring resistance to polymyxin-type antibiotics, as evidenced by increased minimal inhibitory concentrations (MIC) of colistin and polymyxin B in E. coli expressing MCR-1. The pHNSHP45 plasmid, carrying the mcr-1 gene, exhibits efficient transferability to other E. coli strains and significantly elevates polymyxin MIC. Notably, this resistance may not necessitate selective pressure for maintenance within the cell. When introduced into other bacterial species like K. pneumoniae or P. aeruginosa, the plasmid similarly enhances polymyxin MIC. In a murine thigh infection study using an mcr-1-encoding plasmid from a human patient, the plasmid provides in vivo protection against colistin. These findings underscore the crucial role of MCR-1 in mediating resistance to polymyxin antibiotics in various bacterial strains.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

N-His;N-SUMO

A0A0R6L508 (H178-R541)

55632978

HYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYHAKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSCGTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLPKAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAYFKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDVSMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDAITPTLLKLFDVTADKVKDRTAFIR

Approximately 56.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.