METRNL/Meteorin-like Protein, Mouse (His)

METRNL/meteorin-like protein, induced by exercise or cold exposure, promotes energy expenditure. It is produced in skeletal muscle after exercise and in fat tissue after cold exposure and circulates in the body. METRNL/Meteorin-like Protein, Mouse (His) is the recombinant mouse-derived METRNL/Meteorin-like protein, expressed by E. coli , with N-6*His labeled tag.

METRNL/Meteorin-like Protein is a hormone that is induced following exercise or cold exposure, playing a role in promoting energy expenditure. It is produced in skeletal muscle after exercise and in adipose tissue following cold exposure, and can be found in the circulation. METRNL stimulates energy expenditure by promoting browning of white fat depots and improving glucose tolerance. Interestingly, it does not directly act on adipocytes to increase thermogenic gene programs, but rather stimulates various immune cell subtypes to enter the adipose tissue and activate their prothermogenic actions. METRNL also facilitates an eosinophil-dependent increase in IL4 expression and promotes alternative activation of adipose tissue macrophages, which is crucial for the increased expression of thermogenic and anti-inflammatory gene programs in fat. Additionally, METRNL is essential for certain cold-induced thermogenic responses, suggesting its involvement in metabolic adaptations to cold temperatures.

Measured in a cell proliferation assay using SH-SY5Y cells. The ED₅₀ of this effect is 0.193 μg/mL, corresponding to a specific activity is 5.18×10³ units/mg.

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  Measured in a cell proliferation assay using SH-SY5Y cells.  The ED₅₀ for this effect is 0.193 μg/mL, corresponding to a specific activity is 5.18×10³ units/mg.

Mouse

E. coli

N-6*His

Q8VE43-1 (Q46-E311)

210029  [NCBI]

QYSSDLCSWKGSGLTREARSKEVEQVYLRCSAGSVEWMYPTGALIVNLRPNTFSPAQNLTVCIKPFRDSSGANIYLEKTGELRLLVRDIRGEPGQVQCFSLEQGGLFVEATPQQDISRRTTGFQYELMSGQRGLDLHVLSAPCRPCSDTEVLLAICTSDFVVRGFIEDVTHVPEQQVSVIYLRVNRLHRQKSRVFQPAPEDSGHWLGHVTTLLQCGVRPGHGEFLFTGHVHFGEAQLGCAPRFSDFQRMYRKAEEMGINPCEINME

30-33 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.