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NAPG Protein, Human (His)

Cat. No.: HY-P76504
COA Handling Instructions

NAPG Protein is crucial for vesicular transport between the endoplasmic reticulum and Golgi apparatus. Its interactions with RAB11FIP5 and VTI1A indicate involvement in intricate protein networks and precise vesicle movement within the endomembrane system. NAPG's ability to engage with specific partners positions it as a central player in intracellular transport, emphasizing its importance in maintaining cellular organization and function. NAPG Protein, Human (His) is the recombinant human-derived NAPG protein, expressed by E. coli , with N-His labeled tag. The total length of NAPG Protein, Human (His) is 312 a.a., with molecular weight of ~37 KDa.

For research use only. We do not sell to patients.

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Description

NAPG Protein is crucial for vesicular transport between the endoplasmic reticulum and Golgi apparatus. Its interactions with RAB11FIP5 and VTI1A indicate involvement in intricate protein networks and precise vesicle movement within the endomembrane system. NAPG's ability to engage with specific partners positions it as a central player in intracellular transport, emphasizing its importance in maintaining cellular organization and function. NAPG Protein, Human (His) is the recombinant human-derived NAPG protein, expressed by E. coli , with N-His labeled tag. The total length of NAPG Protein, Human (His) is 312 a.a., with molecular weight of ~37 KDa.

Background

NAPG Protein plays a crucial role in facilitating vesicular transport dynamics between the endoplasmic reticulum and the Golgi apparatus. This functional significance is underscored by its interactions with key cellular components, such as RAB11FIP5, indicative of its involvement in intricate protein networks orchestrating membrane trafficking processes. Additionally, NAPG Protein exhibits interactions with VTI1A, further emphasizing its role in coordinating the precise and regulated movement of vesicles within the cellular endomembrane system. The ability of NAPG to engage with specific binding partners positions it as a central player in the complex machinery governing intracellular transport pathways, highlighting its importance in maintaining cellular organization and function.

Species

Human

Source

E. coli

Tag

N-His

Accession

Q99747 (M1-C312)

Gene ID
Molecular Construction
N-term
His
NAPG (M1-C312)
Accession # Q99747
C-term
Synonyms
Gamma-soluble NSF attachment protein; SNAP-gamma; NAPG; SNAPG
Molecular Weight

Approximately 37 kDa.

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

NAPG Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
NAPG Protein, Human (His)
Cat. No.:
HY-P76504
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