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Neurofilament light polypeptide/NEFL, Mouse (His)

Cat. No.: HY-P72305
COA Handling Instructions

Neurofilament light chain polypeptide (NEFL) is a part of neurofilaments that, in cooperation with NEFM and NEFH, form the structural basis of neurons.It maintains neuronal caliber and assembles filamentous networks with PRPH and INA.Neurofilament light polypeptide/NEFL, Mouse (His) is the recombinant mouse-derived Neurofilament light polypeptide/NEFL, expressed by E.coli , with N-His labeled tag.

For research use only. We do not sell to patients.

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2 μg $57 In-stock
10 μg $190 In-stock
50 μg $400 In-stock
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Description

Neurofilament light chain polypeptide (NEFL) is a part of neurofilaments that, in cooperation with NEFM and NEFH, form the structural basis of neurons.It maintains neuronal caliber and assembles filamentous networks with PRPH and INA.Neurofilament light polypeptide/NEFL, Mouse (His) is the recombinant mouse-derived Neurofilament light polypeptide/NEFL, expressed by E.coli , with N-His labeled tag.

Background

Neurofilament light polypeptide (NEFL), a component of neurofilaments, typically collaborates with the intermediate filament proteins NEFM and NEFH to maintain neuronal caliber, forming a structural foundation in neurons. In conjunction with neuronal intermediate filament proteins such as PRPH and INA, NEFL may participate in the assembly of neuronal filamentous networks. In vitro, NEFL demonstrates the ability to form homodimers and engage in heterodimerization with NEFH or NEFM, further forming hetero-oligomers. Additionally, NEFL forms heterodimers with INA. Its interactions extend to regulatory relationships, as evidenced by its association with ARHGEF28 and TRIM2, emphasizing the intricate molecular interplay involved in shaping neuronal structural integrity.

Species

Mouse

Source

E. coli

Tag

N-His

Accession

P08551 (S2-D543)

Gene ID
Molecular Construction
N-term
His
NEFL (S2-D543)
Accession # P08551
C-term
Synonyms
Nefl; Nf68; 68 kDa neurofilament protein; Neurofilament triplet L protein
AA Sequence

SSFGYDPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYSGLQSSSYLMSARSFPAYYTSHVQEEQTEVEETIEATKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGAEEEEAAKDESEDTKEEEEGGEGEEEDTKESEEEEKKEESAGEEQVAKKKD

Molecular Weight

Approximately 68 kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Neurofilament light polypeptide/NEFL, Mouse (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Neurofilament light polypeptide/NEFL, Mouse (His)
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HY-P72305
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