1. Recombinant Proteins
  2. CAR-T Related Proteins CD Antigens
  3. CD314/NKG2D T Cell CD Proteins NK Cell CD Proteins
  4. CD314/NKG2D
  5. NKG2D/CD314 Protein, Mouse (sf9, Fc)

NKG2D is an activating cell surface receptor that associates selectively with DAP10, an YxxM-bearing adaptor protein.NKG2D/CD314 Protein, Mouse (sf9, Fc) is the recombinant mouse-derived NKG2D/CD314 protein, expressed by Sf9 insect cells , with N-hFc labeled tag.

For research use only. We do not sell to patients.

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Description

NKG2D is an activating cell surface receptor that associates selectively with DAP10, an YxxM-bearing adaptor protein.NKG2D/CD314 Protein, Mouse (sf9, Fc) is the recombinant mouse-derived NKG2D/CD314 protein, expressed by Sf9 insect cells , with N-hFc labeled tag.

Background

NKG2D is an activating cell surface receptor that associates selectively with DAP10, an YxxM-bearing adaptor protein. NKG2D enables several functions, including MHC class I protein binding activity, MHC class Ib receptor activity and identical protein binding activity. NKG2D is involved in positive regulation of natural killer cell mediated cytotoxicity. NKG2D acts upstream of or within several processes, including positive regulation of interferon-gamma production, positive regulation of nitric oxide biosynthetic process and response to bacterium. NKG2D is located in external side of plasma membrane[1][2][3].

Species

Mouse

Source

Sf9 insect cells

Tag

N-hFc

Accession

Q4FJM0 (F77-V219)

Gene ID
Molecular Construction
N-term
hFc
NKG2D (F77-V219)
Accession # Q4FJM0
C-term
Synonyms
NKG2-D type II integral membrane protein; NK cell receptor D; CD314; KLRK1; D12S2489E
Molecular Weight

Approximately 44.9 kDa.

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

NKG2D/CD314 Protein, Mouse (sf9, Fc) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Inquiry Information

Product Name:
NKG2D/CD314 Protein, Mouse (sf9, Fc)
Cat. No.:
HY-P76513
Quantity:
MCE Japan Authorized Agent: