1. Recombinant Proteins
  2. Receptor Proteins
  3. Notch family
  4. Notch-2
  5. NOTCH2 Protein, Human (sf9, His)

NOTCH2 Protein, Human (sf9, His)

Cat. No.: HY-P76519
Handling Instructions

NOTCH2 protein is a receptor for ligands such as JAG1, JAG2 and DLL1 and is critical for cell fate determination. Ligand activation promotes cleavage and releases NICD, which forms a transcription activator complex with RBPJ/RBPSUH, affecting cell differentiation, proliferation and apoptosis. NOTCH2 Protein, Human (sf9, His) is the recombinant human-derived NOTCH2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.

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Description

NOTCH2 protein is a receptor for ligands such as JAG1, JAG2 and DLL1 and is critical for cell fate determination. Ligand activation promotes cleavage and releases NICD, which forms a transcription activator complex with RBPJ/RBPSUH, affecting cell differentiation, proliferation and apoptosis. NOTCH2 Protein, Human (sf9, His) is the recombinant human-derived NOTCH2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

NOTCH2 protein functions as a receptor for membrane-bound ligands, including Jagged-1 (JAG1), Jagged-2 (JAG2), and Delta-1 (DLL1), playing a pivotal role in regulating cell-fate determination. Upon ligand activation, NOTCH2 undergoes cleavage, releasing the Notch intracellular domain (NICD), which forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes within the enhancer of split locus. This activation influences cellular differentiation, proliferation, and apoptotic programs. NOTCH2 is implicated in bone remodeling and homeostasis, collaborating with RELA/p65 to enhance NFATc1 promoter activity and positively regulate RANKL-induced osteoclast differentiation. Additionally, it positively regulates self-renewal in liver cancer cells. NOTCH2 exists as a heterodimer composed of a C-terminal fragment (N(TM)) and an N-terminal fragment (N(EC)), likely linked by disulfide bonds. It interacts with various proteins, including transcriptional coactivators MAML1, MAML2, and MAML3, as well as factors such as HIF1AN, TCIM, and FBXW7, implicating its involvement in diverse cellular processes and regulatory pathways. Interactions with MINAR1, NOTCH2NL, MDK, and MINAR2 further contribute to the intricate network of NOTCH2 signaling.

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

Q04721 (L26-Q530)

Gene ID
Molecular Construction
N-term
NOTCH2 (L26-Q530)
Accession # Q04721
His
C-term
Synonyms
AGS2; hN2; Notch homolog 2; Notch2; Notch-2; HJCYS
Molecular Weight

Approximately 55.5 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

NOTCH2 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NOTCH2 Protein, Human (sf9, His)
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HY-P76519
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