1. Recombinant Proteins
  2. Receptor Proteins
  3. Notch family
  4. Notch-4
  5. NOTCH4 Protein, Human (sf9, His)

NOTCH4 Protein, Human (sf9, His)

Cat. No.: HY-P74683
Handling Instructions

NOTCH4 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and is critical for cell fate decisions. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes in the cleavage site enhancer. NOTCH4 Protein, Human (sf9, His) is the recombinant human-derived NOTCH4 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of NOTCH4 Protein, Human (sf9, His) is 637 a.a., with molecular weight of ~66.1 kDa.

For research use only. We do not sell to patients.

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Description

NOTCH4 protein is a receptor for the ligands JAG1, JAG2 and DLL1 and is critical for cell fate decisions. Upon ligand activation, NICD forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes in the cleavage site enhancer. NOTCH4 Protein, Human (sf9, His) is the recombinant human-derived NOTCH4 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of NOTCH4 Protein, Human (sf9, His) is 637 a.a., with molecular weight of ~66.1 kDa.

Background

NOTCH4 protein functions as a receptor for membrane-bound ligands, including Jagged1, Jagged2, and Delta1, crucial for the regulation of cell-fate determination. Upon ligand activation, the released notch intracellular domain (NICD) forms a transcriptional activator complex with RBPJ/RBPSUH, leading to the activation of genes within the enhancer of split locus. Its role extends to influencing differentiation, proliferation, and apoptotic programs, potentially contributing to the regulation of branching morphogenesis in the developing vascular system. Structurally, NOTCH4 exists as a heterodimer, consisting of a C-terminal fragment (N(TM)) and a N-terminal fragment (N(EC)), likely connected by disulfide bonds. The protein interacts with transcriptional coactivators MAML1, MAML2, and MAML3, facilitating their role in modulating downstream transcriptional processes.

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

Q99466 (M1-F637)

Gene ID
Molecular Construction
N-term
NOTCH4 (M1-F637)
Accession # Q99466
His
C-term
Synonyms
Neurogenic locus notch homolog protein 4; hNotch4; INT3
Molecular Weight

Approximately 66.1 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

NOTCH4 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NOTCH4 Protein, Human (sf9, His)
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HY-P74683
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