Nucleoprotein/NP Protein, Influenza B virus (His-SUMO)

Nucleoprotein (NP) protects viral RNA and serves as a replication template. Nucleoprotein/NP Protein, Influenza B virus (His-SUMO) is the recombinant Virus-derived Nucleoprotein/NP protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Nucleoprotein/NP Protein, Influenza B virus (His-SUMO) is 560 a.a., with molecular weight of ~78.0 kDa.

Nucleoprotein/NP Protein plays a pivotal role in the influenza virus life cycle by encapsidating the negative strand viral RNA, shielding it from nucleases and forming the ribonucleoprotein (RNP) complex. This RNP serves as the template for both transcription and replication processes. To initiate an infectious cycle, the RNP must be localized in the host nucleus, a task complicated by its size, preventing diffusion through the nuclear pore complex. NP possesses at least two nuclear localization signals facilitating active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in infection, nuclear export of RNPs is orchestrated by viral proteins NEP interacting with M1, which binds nucleoproteins. There is a possibility that nucleoprotein directly binds to host exportin-1/XPO1, actively participating in RNPs nuclear export. M1's interaction with RNP conceals nucleoprotein's nuclear localization signals, but upon virion acidification driven by the M2 protein, M1 dissociates from the RNP, unveiling nucleoprotein's signals and guiding the RNP to the nucleus. Nucleoprotein homomultimerizes to form the nucleocapsid and may directly bind host exportin-1/XPO1. The intricate protein-RNA contacts involve electrostatic interactions between positively charged residues and the phosphate backbone, along with planar interactions between aromatic side chains and bases.

Virus

E. coli

N-SUMO;N-6*His

P04666 (M1-Y560)

/

MSNMDIDGINTGTIDKTPEEIISGTSGATRPIIRPATLAPPSNKRTRNPSPERATTSSEADVGRKTQKKQTPTEIKKSVYNMVVKLGEFYNQMMVKAGLNDDMERNLIQNAHAVERILLAATDDKKTEFQKKKNARDVKEGKEEIDHNKTGGTFYKMVRDDKTIYFSPIRITFLKEEVKTMYKTTMGSDGFSGLNHIMIGHSQMNDVCFQRSKALKRVGLDPSLISTFAGSTLPRRSGATGVAIKGGGTLVAEAIRFIGRAMADRGLLRDIKAKTAYEKILLNLKNKCSAPQQKALVDQVIGSRNPGIADIEDLTLLARSMVVVRPSVASKVVLPISIYAKIPQLGFNVEEYSMVGYEAMALYNMATPVSILRMGDDAKDKSQLFFMSCFGAAYEDLRVLSALTGTEFKPRSALKCKGFHVPAKEQVEGMGAALMSIKLQFWAPMTRSGGNEVGGDGGSGQISCSPVFAVERPIALSKQAVRRMLSMNIEGRDADVKGNLLKMMNDSMAKKTNGNAFIGKKMFQISDKNKTNPVEIPIKQTIPNFFFGRDTAEDYDDLDY

Approximately 78.0 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1.0 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.