1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. OSM Protein, Mouse (HEK293)

OSM Protein, Mouse (HEK293)

Cat. No.: HY-P7274
COA Handling Instructions

OSM Protein, Mouse (HEK293) is a gp130 cytokine family member, shows homeostatic and proinflammatory activities and can modify extracellular matrix.

For research use only. We do not sell to patients.

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10 μg $75 In-stock
50 μg $210 In-stock
100 μg $360 In-stock
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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

OSM Protein, Mouse (HEK293) is a gp130 cytokine family member, shows homeostatic and proinflammatory activities and can modify extracellular matrix.

Background

Oncostatin M is a gp130 cytokine family member, shows homeostatic and proinflammatory activities and can modify extracellular matrix[1].

Biological Activity

Measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblasts cells. The ED50 this effect is ≤4.975 ng/mL, corresponding to a specific activity is > 2.0101×105 units/mg.

  • Measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblasts cells. The ED50 for this effect is 4.975 ng/mL, corresponding to a specific activity is 2.0101×105 units/mg.
Species

Mouse

Source

HEK293

Tag

Tag Free

Accession

P53347 (A24-R206)

Gene ID
Molecular Construction
N-term
OSM (A24-R206)
Accession # P53347
C-term
Synonyms
rMuOSM; Oncostatin-M
AA Sequence

ANRGCSNSSSQLLSQLQNQANLTGNTESLLEPYIRLQNLNTPDLRAACTQHSVAFPSEDTLRQLSKPHFLSTVYTTLDRVLYQLDALRQKFLKTPAFPKLDSARHNILGIRNNVFCMARLLNHSLEIPEPTQTDSGASRSTTTPDVFNTKIGSCGFLWGYHRFMGSVGRVFREWDDGSTRSRR

Molecular Weight

Approximately 33.25 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PBS.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

OSM Protein, Mouse (HEK293) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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OSM Protein, Mouse (HEK293)
Cat. No.:
HY-P7274
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