1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. Oxidative Stress Responsive Kinase 1 (OXSR1)
  5. OXSR1 Protein, Human (sf9, GST)

OXSR1 Protein, Human (sf9, GST)

Cat. No.: HY-P77119
Handling Instructions

The OXSR1 protein is a key serine/threonine protein kinase in the WNK-SPAK/OSR1 cascade, regulating ion transport, hyperosmotic stress response, and blood pressure. Downstream of WNK kinase, OXSR1 phosphorylates ion cotransporters such as SLC12A1/NKCC2, SLC12A2/NKCC1, and SLC12A3/NCC, regulating their activity. OXSR1 Protein, Human (sf9, GST) is the recombinant human-derived OXSR1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of OXSR1 Protein, Human (sf9, GST) is 527 a.a., with molecular weight of ~80 kDa.

For research use only. We do not sell to patients.

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Description

The OXSR1 protein is a key serine/threonine protein kinase in the WNK-SPAK/OSR1 cascade, regulating ion transport, hyperosmotic stress response, and blood pressure. Downstream of WNK kinase, OXSR1 phosphorylates ion cotransporters such as SLC12A1/NKCC2, SLC12A2/NKCC1, and SLC12A3/NCC, regulating their activity. OXSR1 Protein, Human (sf9, GST) is the recombinant human-derived OXSR1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of OXSR1 Protein, Human (sf9, GST) is 527 a.a., with molecular weight of ~80 kDa.

Background

OXSR1 Protein functions as a key effector serine/threonine-protein kinase in the WNK-SPAK/OSR1 cascade, participating in diverse cellular processes such as ion transport, response to hypertonic stress, and blood pressure regulation. It recognizes and binds proteins with a RFXV motif, acting downstream of WNK kinases to phosphorylate ion cotransporters including SLC12A1/NKCC2, SLC12A2/NKCC1, SLC12A3/NCC, SLC12A5/KCC2, and SLC12A6/KCC3, thereby modulating their activity. OXSR1 mediates regulatory volume increase in response to hyperosmotic stress and regulates NaCl reabsorption in the distal nephron by phosphorylating the thiazide-sensitive Na-Cl cotransporter SLC12A3/NCC. Additionally, it serves as a regulator of angiogenesis in endothelial cells and activates inward rectifier potassium channels KCNJ2/Kir2.1 and KCNJ4/Kir2.3 downstream of WNK1, influencing their membrane localization. OXSR1 also plays a role in phosphorylating various substrates, including RELL1, RELL2, RELT, PAK1, and PLSCR1 in the presence of RELT.

Species

Human

Source

Sf9 insect cells

Tag

N-GST

Accession

O95747 (M1-S527)

Gene ID
Molecular Construction
N-term
GST
OXSR1 (M1-S527)
Accession # O95747
C-term
Synonyms
Serine/threonine-protein kinase OSR1; Oxidative stress-responsive 1 protein; KIAA1101; OSR1
Molecular Weight

Approximately 80 kDa

Purity

Greater than 88% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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OXSR1 Protein, Human (sf9, GST)
Cat. No.:
HY-P77119
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