2. Recombinant Proteins
  3. Immune Checkpoint Proteins CD Antigens
  4. Inhibitory Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins
  5. PD-1
  6. PD-1 Protein, Human (HEK293, Fc)

PD-1 Protein, Human (HEK293, Fc)

Cat. No.: HY-P73344
COA Handling Instructions

PD-1 protein is an inhibitory receptor on T cells that maintains immune tolerance by binding to CD274/PDCD1L1 and CD273/PDCD1LG2. It blocks T cell activation by binding to CD3-TCR and recruiting PTPN11/SHP-2 to dephosphorylate key signaling molecules. PD-1 Protein, Human (HEK293, Fc) is the recombinant human-derived PD-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of PD-1 Protein, Human (HEK293, Fc) is 143 a.a., with molecular weight of 55-66 kDa.

For research use only. We do not sell to patients.

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Description

PD-1 protein is an inhibitory receptor on T cells that maintains immune tolerance by binding to CD274/PDCD1L1 and CD273/PDCD1LG2. It blocks T cell activation by binding to CD3-TCR and recruiting PTPN11/SHP-2 to dephosphorylate key signaling molecules. PD-1 Protein, Human (HEK293, Fc) is the recombinant human-derived PD-1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of PD-1 Protein, Human (HEK293, Fc) is 143 a.a., with molecular weight of 55-66 kDa.
Background

PD-1 protein functions as an inhibitory receptor on antigen-activated T-cells, playing a crucial role in the induction and maintenance of immune tolerance to self. Upon binding to its ligands CD274/PDCD1L1 and CD273/PDCD1LG2, PD-1 delivers inhibitory signals and associates with CD3-TCR in the immunological synapse, directly impeding T-cell activation. This inhibitory action is further executed through the recruitment of PTPN11/SHP-2, leading to the dephosphorylation of key TCR proximal signaling molecules. Exploited by tumors to attenuate anti-tumor immunity, PD-1's interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function. Blockage of the PD-1-mediated pathway has shown promise in reversing the exhausted T-cell phenotype and normalizing the anti-tumor response, providing a rationale for cancer immunotherapy.
Biological Activity

1.Immobilized PD-1 Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind PD-L2 Protein, Human, Recombinant (ECD, His Tag), the EC50 is 45-280ng/mL.
2.Immobilized Recombinant Human PD1 / PDCD1 / CD279 Protein (Fc Tag) at 2 μg/mL (100 μl/well) can bind Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated , the EC50 is 25-75 ng/mL.
3. Labeled biotin to PD-1 Protein, Human, Recombinant (Fc Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PD-1 Protein, Human, Recombinant (Fc Tag), Biotinylated bound to Atezolizumab was 0.3 nM.
Species

Human
Source

HEK293
Tag

C-hFc
Accession

Q15116 (L25-Q167)
Gene ID
Molecular Construction
N-term
PD-1 (L25-Q167)
Accession # Q15116
hFc
C-term
Synonyms
CD279; hPD-1; PDCD1; Programmed cell death 1; SLEB2
Molecular Weight

55-66 kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Solution
Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

N/A.
Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping

Shipping with dry ice.
Documentation

COA (206 KB)

Handling Instructions (2659 KB)

PD-1 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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