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Periostin Protein, Human (781a.a, HEK293, His)

Cat. No.: HY-P74638
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Periostin (POSTN) is a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. POSTN binds to integrins and BMP1 to support cell adhesion, cell migration and the structural integrity of connective tissues. Periostin Protein, Human (781a.a, HEK293, His) is the recombinant human-derived Periostin protein, expressed by HEK293 , with C-His labeled tag. The total length of Periostin Protein, Human (781a.a, HEK293, His) is 781 a.a., with molecular weight of 66-80 kDa.

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  • Biological Activity

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Description

Periostin (POSTN) is a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. POSTN binds to integrins and BMP1 to support cell adhesion, cell migration and the structural integrity of connective tissues. Periostin Protein, Human (781a.a, HEK293, His) is the recombinant human-derived Periostin protein, expressed by HEK293 , with C-His labeled tag. The total length of Periostin Protein, Human (781a.a, HEK293, His) is 781 a.a., with molecular weight of 66-80 kDa.

Background

Periostin (POSTN) is a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. POSTN binds to integrins to support adhesion and migration of epithelial cells, POSTN also enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase, contributing to the structural integrity of connective tissues. POSTN plays a role in cancer stem cell maintenance and metastasis. Lacking of POSTN causes cardiac valve disease, skeletal and dental defects[1][2].

Biological Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to POSTN-His coated plates (20 μg/mL, 100 μL/well), approximately >45% will adhere specifically after 30 minutes at 37°C.

Species

Human

Source

HEK293

Tag

C-His

Accession

B1ALD9 (M1-Q781)

Gene ID
Molecular Construction
N-term
Periostin (M1-Q781)
Accession # B1ALD9
His
C-term
Synonyms
Osteoblast-specific factor 2; OSF-2; POSTN; Periostin
Molecular Weight

66-80 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Periostin Protein, Human (781a.a, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Periostin Protein, Human (781a.a, HEK293, His)
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HY-P74638
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