PKACα Protein, Human (Sf9, GST)

PKACα protein is a key kinase that phosphorylates a variety of substrates, including CDC25B, ABL1, NFKB1, and VASP, affecting a variety of cellular processes, such as cell cycle progression, platelet regulation, and adipogenic differentiation. It also negatively regulates mTORC1 through RPTOR phosphorylation, thereby modulating signaling networks. PKACα Protein, Human (Sf9, GST) is the recombinant human-derived PKACα protein, expressed by Sf9 insect cells , with GST tagged. The total length of PKACα Protein, Human (Sf9, GST) is 350 a.a., .

PKACα, a protein kinase, plays a pivotal role in cellular regulation by phosphorylating a myriad of substrates in both the cytoplasm and the nucleus. Its extensive substrate repertoire includes CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, SOX9, and VASP, among others. Through the phosphorylation of specific substrates, PKACα influences diverse cellular processes such as cell cycle progression, platelet regulation, adipogenic and osteogenic differentiation, chondrogenesis, and tight junction dynamics. Notably, PKACα negatively regulates mTORC1 by phosphorylating RPTOR, acting as a key modulator in the intricate signaling network. Additionally, PKACα exerts regulatory effects on embryonic development, Hedgehog signaling, meiosis resumption, and even REM sleep, showcasing its multifaceted roles in cellular physiology and homeostasis.

Human

Sf9 insect cells

GST

P17612 (G2-F351)

5566

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.