PLA2G2E Protein, Mouse (HEK293, His)

The PLA2G2E protein is a secreted calcium-dependent phospholipase A2 that targets extracellular phospholipids. It hydrolyzes the fatty acyl group at the sn-2 position, releasing unsaturated fatty acids, especially lysophosphatidylethanolamine. PLA2G2E Protein, Mouse (HEK293, His) is the recombinant mouse-derived PLA2G2E protein, expressed by HEK293 , with C-His labeled tag.

PLA2G2E, a secretory calcium-dependent phospholipase A2, predominantly targets extracellular phospholipids and exhibits phospholipase A2 activity by hydrolyzing the ester bond of the fatty acyl group at the sn-2 position of phospholipids. This enzymatic action results in the release of various unsaturated fatty acids, including oleoate, linoleoate, arachidonate, docosahexaenoate, and lysophosphatidylethanolamines, with a preference for lysophosphatidylethanolamines over lysophosphatidylcholines. In response to a high-fat diet, PLA2G2E hydrolyzes minor lipoprotein phospholipids, such as phosphatidylserines, phosphatidylinositols, and phosphatidylglycerols, thereby influencing lipoprotein composition and fat storage in adipose tissue and liver. Operating in an autocrine and paracrine manner, PLA2G2E contributes to lipid remodeling in cellular membranes and the generation of lipid mediators vital for pathogen clearance. Additionally, it acts as a hair follicle phospholipase A2, selectively releasing lysophosphatidylethanolamines and various unsaturated fatty acids in the skin to regulate hair follicle homeostasis. Furthermore, PLA2G2E may play a role in regulating the inflammatory response by releasing arachidonate, a precursor of prostaglandins and leukotrienes. Upon allergen exposure, it potentially participates in the allergic inflammatory response by enhancing leukotriene C4 synthesis and degranulation in mast cells.

Measured by its ability to hydrolyze 80μΜ 1-Hexadecanoyl-2-(1-pyrene-decanoyl)-sn-glycero-3-phosphocholine that incubate at room temperature in kinetic mode for 3 minutes. The specific activity is 124.861 pmol/min/μg.

Mouse

HEK293

C-6*His

Q9QUL3 (N20-C142)

26970  [NCBI]

NLVQFGVMIERMTGKPALQYNDYGCYCGVGGSHWPVDETDWCCHAHDCCYGRLEKLGCDPKLEKYLFSITRDNIFCAGRTACQRHTCECDKRAALCFRHNLNTYNRKYAHYPNKLCTGPTPPC

Approximately 16 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.